Detailed information of TA system TA233672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 84083..85706
• Replicon: plasmid p1559-mcr
• Accession: NZ_CP091548
• Organism: Salmonella enterica strain 1559

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: L4413_RS25175
• Protein ID: WP_140780865.1
• Coordinates: 84381..85706 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: L4413_RS25170
• Protein ID: WP_024136327.1
• Coordinates: 84083..84376 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L4413_RS25145 (L4413_25145)	79955..81196	+	1242	WP_140780866.1	VWA domain-containing protein	-
L4413_RS26280	81287..81418	+	132	WP_000374059.1	hypothetical protein	-
L4413_RS25150 (L4413_25150)	81759..82040	-	282	WP_000077926.1	hypothetical protein	-
L4413_RS25155 (L4413_25155)	82090..82281	-	192	WP_000398480.1	hypothetical protein	-
L4413_RS25160 (L4413_25160)	82373..82714	-	342	WP_001151575.1	hypothetical protein	-
L4413_RS25165 (L4413_25165)	83087..83479	+	393	WP_000341066.1	hypothetical protein	-
L4413_RS25170 (L4413_25170)	84083..84376	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
L4413_RS25175 (L4413_25175)	84381..85706	+	1326	WP_140780865.1	type II toxin-antitoxin system HipA family toxin	Toxin
L4413_RS25180 (L4413_25180)	85767..85973	+	207	WP_000134171.1	hypothetical protein	-
L4413_RS25185 (L4413_25185)	86075..86458	+	384	WP_140780864.1	hypothetical protein	-
L4413_RS25190 (L4413_25190)	86498..87313	+	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
L4413_RS25195 (L4413_25195)	87567..87992	+	426	WP_001043843.1	hypothetical protein	-
L4413_RS25200 (L4413_25200)	88446..88682	+	237	Protein_86	type I restriction-modification system subunit M N-terminal domain-containing protein	-
L4413_RS25205 (L4413_25205)	88737..89036	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / dfrA12 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / mcr-1.1 / qnrS2 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / floR	-	1..271705	271705

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50291.28 Da
• Isoelectric Point: 6.1927

>T233672 WP_140780865.1 NZ_CP091548:84381-85706 [Salmonella enterica]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHVEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T233672 NZ_LM993812:c1874392-1874289 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT233672 WP_024136327.1 NZ_CP091548:84083-84376 [Salmonella enterica]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT233672 NZ_LM993812:1874251-1874522 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1