Detailed information of TA system TA233617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4155057..4155633
• Replicon: chromosome
• Accession: NZ_CP091542
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain S58L2

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: L5Z53_RS20500
• Protein ID: WP_001131963.1
• Coordinates: 4155346..4155633 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: L5Z53_RS20495
• Protein ID: WP_000063142.1
• Coordinates: 4155057..4155359 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5Z53_RS20480 (4151567)	4151567..4153717	+	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-
L5Z53_RS20485 (4153812)	4153812..4154015	+	204	WP_000460616.1	YbdD/YjiX family protein	-
L5Z53_RS20490 (4154026)	4154026..4154982	+	957	WP_000187839.1	GTPase	-
L5Z53_RS20495 (4155057)	4155057..4155359	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
L5Z53_RS20500 (4155346)	4155346..4155633	-	288	WP_001131963.1	BrnT family toxin	Toxin
L5Z53_RS20505 (4155902)	4155902..4156816	-	915	WP_000290512.1	restriction endonuclease	-
L5Z53_RS20510 (4157014)	4157014..4160523	+	3510	WP_001043484.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T233617 WP_001131963.1 NZ_CP091542:c4155633-4155346 [Salmonella enterica subsp. enterica serovar Typhimurium]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T233617 NZ_HG995459:c5568935-5568570 [Pseudomonas sp. PP3]
ATGAAATGGGACGTTGAATACACGGATGAATTTGGCGGGTGGTGGGTTGGCCTAGATGAAAAGGAGCAGGCGTCTATATC
GGCCAGCGTTGACCTGCTGGAACTCTTCGGGCCTGGCTTGGGTTTTCCTCAAAGCAGCGATATCAAAGGCTCGCGGCATG
GAAACCTCCGAGAACTGAGGGTGCAGCACGCGGGGCGTCCTTATCGCGTGCTATACGCGTTTGATCCGCGTCGATGCGCG
TTATTGTTGATCGGTGGCGATAAAACCGGTCAGGACCGCTGGTACCACGAAAATGTCCCGTTTGCAGAAAAGCTGTACGA
CGAACACTTGGAAACACTGCGTAAAGAGGGCCGAAATGATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT233617 WP_000063142.1 NZ_CP091542:c4155359-4155057 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT233617 NZ_HG995459:c5568577-5568272 [Pseudomonas sp. PP3]
ATGGCTAAAAAATTTGCTGAACTTCAGGCTCGCATGTCGCCGGAATCTCGTGCTGATGCTGAGCGTCTGTTCCAACAGCA
CCTGAAAGAGATGCCCCTGCATGAGCTGCGTAAAGCCCAGGAGTTGAGCCAGGAATCGCTAGCCAAAGCGTTACACGTCA
ATCAGGCGGCCATATCCAAAATGGAACGACGCACCGACATGTACATCAGTACATTGCGTAACTACATCCGCGCCATGGGT
GGCGAACTGGAAATTATCGCGACCTTTCCTGAAGGTCAGGTAAAGATCGATAACTTTTCCAACTGA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01