Detailed information of TA system TA233596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 206521..207281
• Replicon: chromosome
• Accession: NZ_CP091542
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain S58L2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q57IH1
• Locus tag: L5Z53_RS00970
• Protein ID: WP_000533909.1
• Coordinates: 206796..207281 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: M7RHS4
• Locus tag: L5Z53_RS00965
• Protein ID: WP_000965886.1
• Coordinates: 206521..206808 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5Z53_RS00940 (201816)	201816..202118	+	303	WP_000605590.1	YsaB family lipoprotein	-
L5Z53_RS00945 (202257)	202257..203168	+	912	WP_001168551.1	glycine--tRNA ligase subunit alpha	-
L5Z53_RS00950 (203178)	203178..205247	+	2070	WP_001291736.1	glycine--tRNA ligase subunit beta	-
L5Z53_RS00955 (205429)	205429..205704	+	276	Protein_189	IS3 family transposase	-
L5Z53_RS00960 (205876)	205876..206343	+	468	WP_000702452.1	GNAT family N-acetyltransferase	-
L5Z53_RS00965 (206521)	206521..206808	+	288	WP_000965886.1	DUF1778 domain-containing protein	Antitoxin
L5Z53_RS00970 (206796)	206796..207281	+	486	WP_000533909.1	GNAT family N-acetyltransferase	Toxin
L5Z53_RS00975 (207653)	207653..208192	-	540	WP_000047149.1	copper-binding periplasmic metallochaperone CueP	-
L5Z53_RS00980 (208366)	208366..208578	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
L5Z53_RS00985 (208867)	208867..209157	-	291	WP_000455790.1	HTH-type transcriptional regulator	-
L5Z53_RS00990 (209596)	209596..210306	+	711	WP_000190524.1	DUF3053 domain-containing protein	-
L5Z53_RS00995 (210356)	210356..211330	-	975	WP_000804674.1	glyoxylate/hydroxypyruvate reductase GhrB	-
L5Z53_RS01000 (211549)	211549..212211	-	663	WP_000747548.1	OmpA family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17703.41 Da
• Isoelectric Point: 9.8719

>T233596 WP_000533909.1 NZ_CP091542:206796-207281 [Salmonella enterica subsp. enterica serovar Typhimurium]
VGRVTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKSSQTQQRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T233596 NZ_HG994851:1929948-1930202 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10950.60 Da
• Isoelectric Point: 4.6811

>AT233596 WP_000965886.1 NZ_CP091542:206521-206808 [Salmonella enterica subsp. enterica serovar Typhimurium]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILETACQAAEKVILDRRVFNFNDEQYEEFINLLDAPVADD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT233596 NZ_HG994851:1929700-1929951 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 7F36
• PDB: 7AK8
• PDB: 5FVJ

Antitoxin

• AlphaFold DB: A0A3V2JDX2