Detailed information of TA system TA233565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 71183..71808
• Replicon: plasmid p537-20_1
• Accession: NZ_CP091535
• Organism: Escherichia coli strain 537-20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L6D13_RS24275
• Protein ID: WP_000911313.1
• Coordinates: 71410..71808 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: L6D13_RS24270
• Protein ID: WP_000450520.1
• Coordinates: 71183..71410 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6D13_RS24270 (71183)	71183..71410	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
L6D13_RS24275 (71410)	71410..71808	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
L6D13_RS24280 (71817)	71817..73970	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
L6D13_RS24285 (74223)	74223..74954	-	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
L6D13_RS24290 (74986)	74986..75483	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iucA / iucB / iucC / iucD / iutA	1..126021	126021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T233565 WP_000911313.1 NZ_CP091535:71410-71808 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T233565 NZ_HG992758:2017652-2018158 [Vibrio sp. B1FLJ16]
TTGGGAATAAGTGCACCAACCCTCCTCACTGATGAGCACCAGCTTAATGCCTTTCAATGTGGCATTGAAGAGCTCGATAC
TTGGTTAAGAAAACAGGCGTTAAAGAGTCAAAAGCGGGGAACCGCGAGGGTGTATGTGGTCAATGACACTGACGCCCATC
AAGTGGTCGGGTACTATGCCATTGCCATGGGATCAGTTTCCAGAGAGCAAGCTTTTAGTTCATTAAGAAGAAATAGTCCA
GATCCCATTCCCATGGTGATTCTAGCCAGACTTGGAGTAGATAACGCCTATCAAGGTCAAGGCATTGCGGCAGGTCTTTT
AAAAGATTGTATCATTCGCTCAGTACAAGCAATGAACGCTGTCGGTGGAGCAGGAATCTTAGTCCATGCTATTGATGGTA
GTGCGCAAACGTTCTACAAGAAATTTGGCTTCAAAGAATCGACGTTTGATCCTTTAGTACTCATGGCAAGGATCTGCGAT
ATCGAAAAATCATTGTCGATAGACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT233565 WP_000450520.1 NZ_CP091535:71183-71410 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT233565 NZ_HG992758:2017395-2017661 [Vibrio sp. B1FLJ16]
ATGGCTACAAAGACAGCCCCAATCAACATGCGAGTACTGCCATCGGTAAGAGACATCATCGATGCCGCAGCAAGTTTAAA
AAAAGTTGATAGAACCGTGTTCATTCAGCAAGCAGCGCTCAATGAAGCGCATAGTATACTGGCAGAACAGCGGGATTTTG
TTCTAGAAGCAAAGGCTTTTGAAGCGTTTGATAACGAACTTCGTGCAGAGCCACAGACTCTTGAAGGTATGAAAGATCTG
TTTAAGAGGAAAGCGCCTTGGGAATAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2