Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4491395..4491892 Replicon chromosome
Accession NZ_CP091534
Organism Escherichia coli strain 537-20

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag L6D13_RS21685 Protein ID WP_001173343.1
Coordinates 4491395..4491568 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag L6D13_RS21690 Protein ID WP_001425728.1
Coordinates 4491596..4491892 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6D13_RS21675 (4488065) 4488065..4489522 + 1458 WP_058915037.1 dipeptide/tripeptide permease DtpC -
L6D13_RS21680 (4489759) 4489759..4491276 + 1518 WP_096934331.1 lysine--tRNA ligase -
L6D13_RS21685 (4491395) 4491395..4491568 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
L6D13_RS21690 (4491596) 4491596..4491892 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
L6D13_RS21695 (4492119) 4492119..4492391 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
L6D13_RS21700 (4492403) 4492403..4492633 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
L6D13_RS21705 (4492814) 4492814..4494445 + 1632 WP_001216487.1 sensor histidine kinase -
L6D13_RS21710 (4494442) 4494442..4495161 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T233560 WP_001173343.1 NZ_CP091534:c4491568-4491395 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T233560 NZ_HG992751:1687080-1687412 [Vibrio sp. B1FIG11]
ATGAAAAGTGTATTTGTCGAATCAACCATATTTGAAAAGTACCGTAATGAATATCTCAGCGATGATGAGTTTAGGCTTTT
TCAAGCAGAGTTAATGTCTAACCCGAAGCAGGGTGACGTGATTCAAGGTACAGGTGGTTTGCGAAAGATTCGAGTTGCAA
GTAAAGGTAAGGGCAAACGTGGTGGTTCTCGTGTTATCTATTACTTCCTCGATGAAAAGCGTCGTTTCTACTTGCTGACG
ATTTACGGCAAGAATGAAATGTCCGACTTAACCGCAGACCAAAAGAAACAACTAAAGGCTTTTATGGAGGCGTGGCGTAA
TGAGCAATCGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT233560 WP_001425728.1 NZ_CP091534:c4491892-4491596 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT233560 NZ_HG992751:1687399-1687713 [Vibrio sp. B1FIG11]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGTTCAGCACTTGTTGAGGCTAAAGAGCATTCAGAGGGCAAGCTTACCCT
TAAAACTCATCAAGTTAACGACATCAGTGAGTTAAATATCTCACCTGATGAAATCGTGAGTATTCGAGAGCAATTTAATA
TGTCTCGTGGTGTGTTTGCTCGTTTGTTGCACACATCATCTCGTACGTTAGAAAACTGGGAACAAGGGCGTAGCGCACCA
AATGGGCAAGCAGTAACCCTTCTAAAGTTGGTTCAGCGTCACCCAGAAACTCTGTCGCACATCGCTGAGCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References