Detailed information of TA system TA233540

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 866002..866837
• Replicon: chromosome
• Accession: NZ_CP091534
• Organism: Escherichia coli strain 537-20

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1C0Y405
• Locus tag: L6D13_RS04195
• Protein ID: WP_001774607.1
• Coordinates: 866002..866379 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M2U6P5
• Locus tag: L6D13_RS04200
• Protein ID: WP_001774606.1
• Coordinates: 866469..866837 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6D13_RS04165 (861410)	861410..862387	+	978	WP_096934255.1	type II secretion system minor pseudopilin GspK	-
L6D13_RS04170 (862384)	862384..863562	+	1179	WP_000094970.1	type II secretion system protein GspL	-
L6D13_RS04175 (863564)	863564..864100	+	537	WP_000942786.1	GspM family type II secretion system protein YghD	-
L6D13_RS04180 (864381)	864381..865223	-	843	WP_032152755.1	DUF4942 domain-containing protein	-
L6D13_RS04185 (865308)	865308..865505	-	198	WP_032152719.1	DUF957 domain-containing protein	-
L6D13_RS04190 (865517)	865517..866005	-	489	WP_001774608.1	DUF5983 family protein	-
L6D13_RS04195 (866002)	866002..866379	-	378	WP_001774607.1	TA system toxin CbtA family protein	Toxin
L6D13_RS04200 (866469)	866469..866837	-	369	WP_001774606.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
L6D13_RS04205 (866887)	866887..867531	-	645	WP_001774605.1	hypothetical protein	-
L6D13_RS04210 (867550)	867550..867771	-	222	WP_000692312.1	DUF987 domain-containing protein	-
L6D13_RS04215 (867834)	867834..868310	-	477	WP_001297237.1	RadC family protein	-
L6D13_RS04220 (868326)	868326..868805	-	480	WP_032152717.1	antirestriction protein	-
L6D13_RS04225 (869144)	869144..869962	-	819	WP_016238867.1	DUF932 domain-containing protein	-
L6D13_RS04230 (870061)	870061..870294	-	234	WP_001278287.1	DUF905 family protein	-
L6D13_RS04235 (870300)	870300..870977	-	678	WP_001097305.1	hypothetical protein	-
L6D13_RS04240 (871125)	871125..871805	-	681	WP_032082725.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	864254..907694	43440

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14218.20 Da
• Isoelectric Point: 7.8045

>T233540 WP_001774607.1 NZ_CP091534:c866379-866002 [Escherichia coli]
MKTLPDTHVREASRCPPPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDTVNFLVEKYALIRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T233540 NZ_HG992338:c4865079-4864945 [Xanthomonas arboricola pv. corylina]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGGTGTTGTCGGTCGGTATGCTGTCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCTGCGCGCAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.31 Da
• Isoelectric Point: 5.8746

>AT233540 WP_001774606.1 NZ_CP091534:c866837-866469 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT233540 NZ_HG992338:c4865314-4865162 [Xanthomonas arboricola pv. corylina]
ATGAAGCGACTGCTGACACTGATGGTACTGGGCCTGTTTTCGGCCGGCGTGATGACTGGCTGCAACACCATGGCCGGCGC
TGGCAAGGACATGCAGGGTGCAGGTGAGAAGGTCGAAAAGAAGGCCGACAATTGCAGCGACGGTAAGTGCTGA

Structures

Toxin

• AlphaFold DB: A0A1C0Y405

Antitoxin

• AlphaFold DB: A0A1M2U6P5