Detailed information of TA system TA233533

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 4296011..4296711
• Replicon: chromosome
• Accession: NZ_CP091527
• Organism: Klebsiella sp. PCX

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: GQR96_RS20420
• Protein ID: WP_236948518.1
• Coordinates: 4296011..4296334 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GQR96_RS20425
• Protein ID: WP_236948519.1
• Coordinates: 4296355..4296711 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQR96_RS20400 (4291096)	4291096..4292115	+	1020	WP_004205333.1	aldo/keto reductase	-
GQR96_RS20405 (4292148)	4292148..4292951	+	804	WP_004205332.1	carboxymuconolactone decarboxylase family protein	-
GQR96_RS20410 (4293199)	4293199..4294116	-	918	WP_004205331.1	LysR family transcriptional regulator	-
GQR96_RS20415 (4294246)	4294246..4295634	+	1389	WP_049116114.1	MFS transporter	-
GQR96_RS20420 (4296011)	4296011..4296334	-	324	WP_236948518.1	TA system toxin CbtA family protein	Toxin
GQR96_RS20425 (4296355)	4296355..4296711	-	357	WP_236948519.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GQR96_RS20430 (4296737)	4296737..4296958	-	222	WP_012543032.1	DUF987 domain-containing protein	-
GQR96_RS20435 (4296955)	4296955..4297497	-	543	WP_236948520.1	DNA repair protein RadC	-
GQR96_RS20440 (4297583)	4297583..4298014	-	432	WP_048294092.1	antirestriction protein	-
GQR96_RS20445 (4298183)	4298183..4298353	-	171	Protein_3984	DUF905 family protein	-
GQR96_RS20450 (4298350)	4298350..4299168	-	819	WP_048294090.1	DUF932 domain-containing protein	-
GQR96_RS20455 (4299253)	4299253..4299735	-	483	WP_228136721.1	hypothetical protein	-
GQR96_RS20460 (4299740)	4299740..4300147	-	408	WP_048294088.1	hypothetical protein	-
GQR96_RS20465 (4300324)	4300324..4301211	-	888	WP_048294087.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-106)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12238.08 Da
• Isoelectric Point: 6.2092

>T233533 WP_236948518.1 NZ_CP091527:c4296334-4296011 [Klebsiella sp. PCX]
MKFLPATNMRAAKPCQSPVTIWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRTDRRGF
SWQEQTSYLTIIDIMRARCDLGLLNHN

Nucleotide

• Length: 324 bp

>T233533 NZ_HG969192:2251175-2251459 [Bartonella tribocorum]
ATGAAAACAACCCCATTTAAACCAGAAGAATATCTTGAAGACATTGAAGTACAACAAGCATTTCTCAATGAAGCCTTCAA
AACTGGTGATGCCGCTCATATAGCGGATGCCATTGGTATCGTTGCTCGCTCTCAAAATATGCGTGCTTTAGCAAAAGAAA
CCAATCGCGAACGCAGTGGATTATATCGCTCCTTAAGTAAAACGGGTGACCCCAAGCTTTCTACTTTAGTCGCTGTCTTA
TCTGCCCTTAACCTGCAACTCTTTGTACAGTCTTCTACTTCATAA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13120.77 Da
• Isoelectric Point: 5.9520

>AT233533 WP_236948519.1 NZ_CP091527:c4296711-4296355 [Klebsiella sp. PCX]
MNNHSESGDKPENPACQQWGLKSTITPCFGARLVQEGSRVHFLADRAGFNGTFSDEHALRLDQAFPLILKQLELMLTSGE
LSPQHQHCVTLYYNGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 357 bp

>AT233533 NZ_HG969192:2250886-2251194 [Bartonella tribocorum]
ATGATATTCATTCATAAAACGATAGAATTTGATAATTGGCTTAAGAAACTCAAAGATAAAAAGGCTAAAGCGATTATTCT
TCAACGTGTTGTACGCTTAAAACAAGGACTTTTGGGTGATGTTAAGTTCTTCAATAGCATAGGTGAAGTACGCATCCACT
ATGGTGCTGGTTACAGAATTTATTTTACCCAAAAAGGCTCTGATTTTATCCTTTTGTTATGTGGTGGAGATAAATCAACG
CAGCAAAGGGATATCGAACAAGCGTTAAAGTTAAAAGAGGAGTACAGTGATGAAAACAACCCCATTTAA