Detailed information of TA system TA233520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2783730..2784259
• Replicon: chromosome
• Accession: NZ_CP091525
• Organism: Staphylococcus aureus strain N09CSA16

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L4X60_RS13765
• Protein ID: WP_000621175.1
• Coordinates: 2783897..2784259 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: L4X60_RS13760
• Protein ID: WP_000948331.1
• Coordinates: 2783730..2783900 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L4X60_RS13730 (2778767)	2778767..2779327	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
L4X60_RS13735 (2779535)	2779535..2780014	+	480	WP_001287087.1	hypothetical protein	-
L4X60_RS13740 (2780007)	2780007..2781590	+	1584	WP_001294626.1	PH domain-containing protein	-
L4X60_RS13745 (2781577)	2781577..2782068	+	492	WP_001205912.1	PH domain-containing protein	-
L4X60_RS13750 (2782072)	2782072..2782431	+	360	WP_000581197.1	holo-ACP synthase	-
L4X60_RS13755 (2782497)	2782497..2783645	+	1149	WP_001281154.1	alanine racemase	-
L4X60_RS13760 (2783730)	2783730..2783900	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
L4X60_RS13765 (2783897)	2783897..2784259	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L4X60_RS13770 (2784608)	2784608..2785609	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
L4X60_RS13775 (2785728)	2785728..2786054	+	327	WP_001052491.1	anti-sigma factor antagonist	-
L4X60_RS13780 (2786056)	2786056..2786535	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
L4X60_RS13785 (2786510)	2786510..2787280	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T233520 WP_000621175.1 NZ_CP091525:2783897-2784259 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T233520 NZ_HG969192:c434488-434216 [Bartonella tribocorum]
ATGCTGATACCCGTCCGCTCAAGACAATTTAAACGTGACGTGCGCAAAGCGGAAAAACGCGGTAAAAATATGAATAAGCT
GCGTACACTATTGAGTCTTCTAATTGAAGAAAAGCCATTACCAAGACATTACTGTGATCATCCACTAAAAGGCGATTGGG
TAGGATATCATGATATTCATATCGAACCAGATTGGCTGCTTATTTATAGAATAGAAGGAGGTAAATTACATCTTATTCGT
ACAGGAACCCACTCTGATCTTTTCAAAGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT233520 WP_000948331.1 NZ_CP091525:2783730-2783900 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT233520 NZ_HG969192:c434747-434490 [Bartonella tribocorum]
ATGAAAACCGATTCTGTTGTTCGCGCTCGCATTCCTGCTGAAATGAAAAAACAAGCGATGATTACTTTAGAACGTATGGG
ATTAAGTGCATCAGACCTTATCCGCATAACATTTTTGCGTGTCGCTGAAGAAGGTTGTTTGCCTTTTGATCTTAAAGTAC
CTAATAGTGTCACACGTAAAGCTATAAAGGAATTAGATGAAGGTAAAGGTAAGACATTTAAAAACGCTGATGCCCTTTTT
AAGGATTTAGGCATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1