Detailed information of TA system TA233519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2762483..2762997
• Replicon: chromosome
• Accession: NZ_CP091525
• Organism: Staphylococcus aureus strain N09CSA16

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: L4X60_RS13665
• Protein ID: WP_000581792.1
• Coordinates: 2762788..2762997 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: L4X60_RS13660
• Protein ID: WP_000138932.1
• Coordinates: 2762483..2762776 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L4X60_RS13640 (2758019)	2758019..2759338	+	1320	WP_001557163.1	ISL3-like element IS1181 family transposase	-
L4X60_RS13645 (2759341)	2759341..2759412	+	72	Protein_2654	hypothetical protein	-
L4X60_RS13650 (2760155)	2760155..2760802	-	648	WP_001187630.1	HD domain-containing protein	-
L4X60_RS13655 (2760830)	2760830..2762317	-	1488	WP_254241334.1	cardiolipin synthase	-
L4X60_RS13660 (2762483)	2762483..2762776	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
L4X60_RS13665 (2762788)	2762788..2762997	+	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
L4X60_RS13670 (2763125)	2763125..2763262	+	138	WP_000828354.1	Lmo0850 family protein	-
L4X60_RS13675 (2763526)	2763526..2764728	-	1203	WP_001109939.1	FtsW/RodA/SpoVE family cell cycle protein	-
L4X60_RS13680 (2765046)	2765046..2766116	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
L4X60_RS13685 (2766131)	2766131..2767489	+	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2758019..2759338	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T233519 WP_000581792.1 NZ_CP091525:2762788-2762997 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T233519 NZ_HG969192:398046-398360 [Bartonella tribocorum]
ATGATACATAAAAGAACGCATAGAGGTGATTTGGTGATTGAATCTTTTGCGGATAAGCGGTGTAAAGATCTTTTAGAAGG
CAAGACACCCAAAGGTTTTCCTGCGACATTGGTACGCATAGCACAAAGAAAATTGTTTATGCTCGATAAAGCAGTTGATC
TTAAAGATTTGCGCAGTCCTCCAGGAAATCATTTAGAAGCATTGAAAGGAGAGCGTAAAGGTCAATATTCTATTCGTATT
AATAACCAGTTTCGTCTTTGTTTTGAATGGCGTACTAATGGTGCCTATGAAGTTGAAATTGTAGATTATCATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT233519 WP_000138932.1 NZ_CP091525:2762483-2762776 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT233519 NZ_HG969192:398377-398673 [Bartonella tribocorum]
ATGAAAAATTATATTGCAATACATCCTGGAGAAATTTTACGGGAGGAATATTTAAAAGAATATGCTCTTTCTGCTTATGC
TCTTGCAAAAGCATTGAATGTTCCACGCACGAGGATAGAACGTATTATTGCGGAAAATAGCCCAATAACTCCTGATACCG
CTCTCAGACTGGCTTCTTTTTTTGATACGACAGCTGAGTTCTGGCTTAATATGCAAGCTGCTTATGATGTTCATATTTTA
CAAGCCGAAAAAGCAGATGAATTTGCCAAAATTAATAAATTTGAATGTAGAACTTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13