Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 53023..53381 Replicon chromosome
Accession NC_011742
Organism Escherichia coli S88

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag ECS88_RS27605 Protein ID WP_001332330.1
Coordinates 53259..53381 (+) Length 41 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag ECS88_RS01110 Protein ID WP_000125566.1
Coordinates 53023..53256 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECS88_RS01085 48096..48383 + 288 WP_000203741.1 ferredoxin-like protein FixX -
ECS88_RS01090 48442..49773 + 1332 WP_001183207.1 MFS transporter -
ECS88_RS01095 49881..50411 + 531 WP_000600749.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
ECS88_RS01100 50404..52266 + 1863 WP_000377122.1 glutathione-regulated potassium-efflux system protein KefC -
ECS88_RS01105 52458..52937 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
ECS88_RS01110 53023..53256 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
ECS88_RS27605 53259..53381 + 123 WP_001332330.1 CcdB family protein Toxin
ECS88_RS27610 53422..53574 + 153 WP_146692624.1 CcdB family protein -
ECS88_RS01120 53571..54419 - 849 WP_000257201.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
ECS88_RS01125 54426..54803 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
ECS88_RS01130 54806..55627 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
ECS88_RS01135 55624..56613 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
ECS88_RS01140 56613..57899 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-39)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 41 a.a.        Molecular weight: 4563.34 Da        Isoelectric Point: 10.9266

>T23350 WP_001332330.1 NC_011742:53259-53381 [Escherichia coli S88]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Download         Length: 123 bp

>T23350 NC_011742:53259-53381 [Escherichia coli S88]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTGTTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT23350 WP_000125566.1 NC_011742:53023-53256 [Escherichia coli S88]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT23350 NC_011742:53023-53256 [Escherichia coli S88]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References