Detailed information of TA system TA233499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2755672..2756201
• Replicon: chromosome
• Accession: NZ_CP091523
• Organism: Staphylococcus aureus strain N12HSA28

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L5471_RS13545
• Protein ID: WP_000621175.1
• Coordinates: 2755839..2756201 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: L5471_RS13540
• Protein ID: WP_000948331.1
• Coordinates: 2755672..2755842 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5471_RS13510 (2750708)	2750708..2751268	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
L5471_RS13515 (2751477)	2751477..2751956	+	480	WP_001287078.1	hypothetical protein	-
L5471_RS13520 (2751949)	2751949..2753532	+	1584	WP_001294626.1	PH domain-containing protein	-
L5471_RS13525 (2753519)	2753519..2754010	+	492	WP_001205912.1	PH domain-containing protein	-
L5471_RS13530 (2754014)	2754014..2754373	+	360	WP_000581197.1	holo-ACP synthase	-
L5471_RS13535 (2754439)	2754439..2755587	+	1149	WP_001281154.1	alanine racemase	-
L5471_RS13540 (2755672)	2755672..2755842	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
L5471_RS13545 (2755839)	2755839..2756201	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L5471_RS13550 (2756550)	2756550..2757551	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
L5471_RS13555 (2757670)	2757670..2757996	+	327	WP_001052491.1	anti-sigma factor antagonist	-
L5471_RS13560 (2757998)	2757998..2758477	+	480	WP_001190830.1	anti-sigma B factor RsbW	-
L5471_RS13565 (2758452)	2758452..2759222	+	771	WP_001041108.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T233499 WP_000621175.1 NZ_CP091523:2755839-2756201 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T233499 NZ_HG965802:812278-812610 [Bartonella henselae]
ATGAAGCGGGGCGAAATTTGGCTTGTATCTCTTGACCCAACTTCAGGCTATGAACAAAAAGGAACGCGTCCTGTATTGAT
TGTATCACCAGAGGCGTTTAATCGTGTCACGAAAACACCCATTGTCTTACCCATTACAAGTGGAGGAAATTTTTCTCGAA
CCGCAGGTTTCGCTGTCTCATTGGTAGGAACAGGATTGCATACAACAGGTGTGATACGTTGTGACCAGCCACGTGCTCTG
GATATAGCAGCGCGTCAGGGTAAGAAAATGGAAACAGTTCCCCCCATAATTATGAATGAAGTTCTTGCTAAGCTATCAAC
ATTTCTCACATAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT233499 WP_000948331.1 NZ_CP091523:2755672-2755842 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT233499 NZ_HG965802:812021-812278 [Bartonella henselae]
ATGCACACAACAAAATTACGTAAAGTTGGAGGGTCTGTTATGCTCTCTATACCGCCGGCATTGCTTGATGTTCTTCATCT
TACTGAGAACACGCAAGTTGGTTTGGCTGTTGATAATGGACAATTGGTTGTGAAGCCACAGACATTTCCAAGTTACACTC
TTGATGAATTGTTAGCTCAATGTCATCTTTCAGATGATTTCTCTGATGAGGATATGGAGTGGTTTGATGCTAAACCTGTT
GGTAGAGAGCTCTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1