Detailed information of TA system TA233489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1234625..1235102
• Replicon: chromosome
• Accession: NZ_CP091523
• Organism: Staphylococcus aureus strain N12HSA28

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: L5471_RS06205
• Protein ID: WP_000052076.1
• Coordinates: 1234914..1235102 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: L5471_RS06200
• Protein ID: WP_001059690.1
• Coordinates: 1234625..1234885 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5471_RS06165 (1230020)	1230020..1230736	-	717	WP_000150012.1	type I 3-dehydroquinate dehydratase	-
L5471_RS06170 (1230884)	1230884..1231306	+	423	WP_000974464.1	organic hydroperoxide resistance protein	-
L5471_RS06175 (1231705)	1231705..1232199	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
L5471_RS06180 (1232354)	1232354..1232971	+	618	WP_000255544.1	LysE/ArgO family amino acid transporter	-
L5471_RS06185 (1233044)	1233044..1233625	-	582	WP_001140696.1	histidine phosphatase family protein	-
L5471_RS06190 (1233849)	1233849..1234085	+	237	WP_000368687.1	hypothetical protein	-
L5471_RS06195 (1234082)	1234082..1234285	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
L5471_RS06200 (1234625)	1234625..1234885	+	261	WP_001059690.1	hypothetical protein	Antitoxin
L5471_RS06205 (1234914)	1234914..1235102	+	189	WP_000052076.1	hypothetical protein	Toxin
L5471_RS06210 (1235290)	1235290..1235859	+	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
L5471_RS06215 (1235947)	1235947..1236231	+	285	WP_001004401.1	hypothetical protein	-
L5471_RS06220 (1236293)	1236293..1236511	+	219	WP_000005886.1	hypothetical protein	-
L5471_RS06225 (1237007)	1237007..1237207	-	201	WP_001059082.1	cold-shock protein	-
L5471_RS06230 (1237564)	1237564..1238250	-	687	WP_001793574.1	thermonuclease family protein	-
L5471_RS06235 (1238591)	1238591..1239112	-	522	WP_000824464.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T233489 WP_000052076.1 NZ_CP091523:1234914-1235102 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T233489 NZ_HG941718:5055662-5055949 [Escherichia coli O25b:H4-ST131]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT233489 WP_001059690.1 NZ_CP091523:1234625-1234885 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT233489 NZ_HG941718:5055936-5056238 [Escherichia coli O25b:H4-ST131]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56