Detailed information of TA system TA233485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_XRE(antitoxin)
• Location: 529215..530012
• Replicon: chromosome
• Accession: NZ_CP091523
• Organism: Staphylococcus aureus strain N12HSA28

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A641AAS6
• Locus tag: L5471_RS02810
• Protein ID: WP_000525006.1
• Coordinates: 529215..529676 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L5471_RS02815
• Protein ID: WP_001260486.1
• Coordinates: 529689..530012 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5471_RS02785 (526162)	526162..527367	-	1206	WP_000264747.1	site-specific integrase	-
L5471_RS02790 (527493)	527493..528107	+	615	WP_000191459.1	hypothetical protein	-
L5471_RS02795 (528104)	528104..528250	-	147	WP_234722993.1	hypothetical protein	-
L5471_RS02800 (528340)	528340..528735	-	396	WP_000449655.1	hypothetical protein	-
L5471_RS02805 (528764)	528764..529198	-	435	WP_001226136.1	hypothetical protein	-
L5471_RS02810 (529215)	529215..529676	-	462	WP_000525006.1	hypothetical protein	Toxin
L5471_RS02815 (529689)	529689..530012	-	324	WP_001260486.1	helix-turn-helix transcriptional regulator	Antitoxin
L5471_RS02820 (530176)	530176..530424	+	249	WP_000272861.1	helix-turn-helix domain-containing protein	-
L5471_RS02825 (530440)	530440..530685	+	246	WP_001025401.1	DUF2829 domain-containing protein	-
L5471_RS02830 (530654)	530654..531019	-	366	WP_001128433.1	hypothetical protein	-
L5471_RS02835 (531074)	531074..531289	+	216	WP_001036301.1	hypothetical protein	-
L5471_RS02840 (531314)	531314..531577	+	264	WP_001124160.1	helix-turn-helix domain-containing protein	-
L5471_RS02845 (531590)	531590..531751	+	162	WP_001285955.1	DUF1270 family protein	-
L5471_RS02850 (531830)	531830..532153	+	324	WP_000175000.1	hypothetical protein	-
L5471_RS02855 (532168)	532168..532530	+	363	WP_000279446.1	hypothetical protein	-
L5471_RS02860 (532527)	532527..533693	+	1167	WP_000762551.1	DUF2800 domain-containing protein	-
L5471_RS02865 (533719)	533719..534276	+	558	WP_000645042.1	DUF2815 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-65)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 18118.47 Da
• Isoelectric Point: 4.6915

>T233485 WP_000525006.1 NZ_CP091523:c529676-529215 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVYRYKEI

Nucleotide

• Length: 462 bp

>T233485 NZ_HG941718:4768238-4768411 [Escherichia coli O25b:H4-ST131]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12427.10 Da
• Isoelectric Point: 9.3811

>AT233485 WP_001260486.1 NZ_CP091523:c530012-529689 [Staphylococcus aureus]
MRTSAEIGKLIKQLRKENNVNLTDFATKIGVNKSTLSRYENGSRKIPMEDIAEIANALKVTPEYLLLKNRQPENEVQHRA
AHLEGELTDDEWQRVLDYADYIRSKRK

Nucleotide

• Length: 324 bp

>AT233485 NZ_HG941718:4767914-4768210 [Escherichia coli O25b:H4-ST131]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A641AAS6