Detailed information of TA system TA233474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 73957..74585
• Replicon: chromosome
• Accession: NZ_CP091522
• Organism: Neisseria sp. Dent CA1/247

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LVJ85_RS00330
• Protein ID: WP_115134051.1
• Coordinates: 74184..74585 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3S4ZDA2
• Locus tag: LVJ85_RS00325
• Protein ID: WP_004284447.1
• Coordinates: 73957..74181 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ85_RS00305 (LVJ85_00305)	70204..70671	+	468	WP_244740467.1	GNAT family N-acetyltransferase	-
LVJ85_RS00310 (LVJ85_00310)	70757..71266	+	510	WP_244740469.1	hypothetical protein	-
LVJ85_RS00315 (LVJ85_00315)	71563..73095	+	1533	WP_182073206.1	IS21 family transposase	-
LVJ85_RS00320 (LVJ85_00320)	73085..73810	+	726	WP_244738480.1	IS21-like element helper ATPase IstB	-
LVJ85_RS00325 (LVJ85_00325)	73957..74181	+	225	WP_004284447.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ85_RS00330 (LVJ85_00330)	74184..74585	+	402	WP_115134051.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LVJ85_RS00335 (LVJ85_00335)	74795..75964	+	1170	WP_244740472.1	benzoate/H(+) symporter BenE family transporter	-
LVJ85_RS00340 (LVJ85_00340)	76212..76931	+	720	WP_244740474.1	S-methyl-5'-thioinosine phosphorylase	-
LVJ85_RS00345 (LVJ85_00345)	76999..77580	+	582	WP_244740476.1	molybdopterin adenylyltransferase	-
LVJ85_RS00350 (LVJ85_00350)	77906..79156	+	1251	WP_244740479.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pilU / pilT	115..157241	157126
	-	flank	IS/Tn	-	-	73085..73810	725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14825.11 Da
• Isoelectric Point: 7.9466

>T233474 WP_115134051.1 NZ_CP091522:74184-74585 [Neisseria sp. Dent CA1/247]
MLRYMLDTNICIYTIKNNPAAVREKFQQHQHHMCISSIVLTELLYGAEKSSNPAKSLALIEGMAARLEVLNFDETAAAHA
AEIRADLARKGTPIGHYDVLIAGHARSRGLILVSNNLREFERVAGLRLENWAV

Nucleotide

• Length: 402 bp

>T233474 NZ_HG941718:c2289636-2289385 [Escherichia coli O25b:H4-ST131]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8436.55 Da
• Isoelectric Point: 4.6721

>AT233474 WP_004284447.1 NZ_CP091522:73957-74181 [Neisseria sp. Dent CA1/247]
MAQATLFKSNQTQAVRLPKAVAFPDDVKKVEVVVLGKSRLLTPSENLWDDWFAQLPQADFPDREPSFQAERESF

Nucleotide

• Length: 225 bp

>AT233474 NZ_HG941718:c2289388-2289134 [Escherichia coli O25b:H4-ST131]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S4ZDA2