Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) VapXD/-
Location 134397..134843 Replicon chromosome
Accession NZ_CP091519
Organism Alysiella crassa strain S6

Toxin (Protein)


Gene name VapD Uniprot ID A0A376BM83
Locus tag LVJ80_RS00670 Protein ID WP_034293097.1
Coordinates 134397..134648 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name VapX Uniprot ID A0A376BME1
Locus tag LVJ80_RS00675 Protein ID WP_034293095.1
Coordinates 134652..134843 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ80_RS00660 (LVJ80_00660) 131371..133269 - 1899 WP_244773246.1 DNA methyltransferase -
LVJ80_RS00665 (LVJ80_00665) 133357..134343 - 987 WP_034293099.1 lipoyl synthase -
LVJ80_RS00670 (LVJ80_00670) 134397..134648 - 252 WP_034293097.1 virulence factor Toxin
LVJ80_RS00675 (LVJ80_00675) 134652..134843 - 192 WP_034293095.1 DUF5397 family protein Antitoxin
LVJ80_RS00680 (LVJ80_00680) 134934..135209 - 276 WP_034293093.1 hypothetical protein -
LVJ80_RS00685 (LVJ80_00685) 135275..135877 - 603 WP_034293091.1 lipoyl(octanoyl) transferase LipB -
LVJ80_RS00690 (LVJ80_00690) 135879..136148 - 270 WP_034293089.1 DUF493 family protein -
LVJ80_RS00695 (LVJ80_00695) 136660..139152 + 2493 WP_084693552.1 NAD-dependent DNA ligase LigA -
LVJ80_RS00700 (LVJ80_00700) 139182..139544 + 363 WP_051968514.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Virulence-associated protein D / CRISPR associated protein Cas2

(1-78)

Antitoxin

Protein of unknown function DUF5397

(2-62)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9533.76 Da        Isoelectric Point: 7.1990

>T233468 WP_034293097.1 NZ_CP091519:c134648-134397 [Alysiella crassa]
MYAISFDLVVSDTQNAHPKGATLHQYGFQRVQGSLYVNDNEDMANLFSAIQALKSLAWFPTSVRDIRAFRIEQWSDFTKF
VKT

Download         Length: 252 bp

>T233468 NZ_HG941718:538457-538759 [Escherichia coli O25b:H4-ST131]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 6903.74 Da        Isoelectric Point: 4.0824

>AT233468 WP_034293095.1 NZ_CP091519:c134843-134652 [Alysiella crassa]
MINQDNIPTGEVRTFGAFGVPYLVGKAEQELPNGDILVGIELLESGEHEQYPLSHLLQDPKAN

Download         Length: 192 bp

>AT233468 NZ_HG941718:538795-539136 [Escherichia coli O25b:H4-ST131]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376BM83

Antitoxin

Source ID Structure
AlphaFold DB A0A376BME1

References