Detailed information of TA system TA233466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 647148..647698
• Replicon: chromosome
• Accession: NZ_CP091518
• Organism: Kingella kingae strain 4177/66

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F5S5C3
• Locus tag: LVJ79_RS03505
• Protein ID: WP_003785639.1
• Coordinates: 647429..647698 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F5S5C2
• Locus tag: LVJ79_RS03500
• Protein ID: WP_003785638.1
• Coordinates: 647148..647432 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ79_RS03465 (LVJ79_03465)	643279..644136	-	858	WP_003785631.1	tyrosine-type recombinase/integrase	-
LVJ79_RS03470 (LVJ79_03470)	644199..644552	+	354	WP_003785632.1	phage holin family protein	-
LVJ79_RS03475 (LVJ79_03475)	644533..645063	+	531	WP_003785633.1	glycoside hydrolase family 108 protein	-
LVJ79_RS03480 (LVJ79_03480)	645060..645377	+	318	WP_003785634.1	hypothetical protein	-
LVJ79_RS03485 (LVJ79_03485)	645583..645801	+	219	WP_003785635.1	TraR/DksA C4-type zinc finger protein	-
LVJ79_RS03490 (LVJ79_03490)	645788..646219	+	432	WP_003785636.1	phage tail protein	-
LVJ79_RS03495 (LVJ79_03495)	646223..646957	+	735	WP_003785637.1	phage virion morphogenesis protein	-
LVJ79_RS03500 (LVJ79_03500)	647148..647432	+	285	WP_003785638.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LVJ79_RS03505 (LVJ79_03505)	647429..647698	+	270	WP_003785639.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LVJ79_RS03510 (LVJ79_03510)	647857..648465	+	609	WP_003785640.1	phage baseplate assembly protein V	-
LVJ79_RS03515 (LVJ79_03515)	648462..648797	+	336	WP_003785641.1	GPW/gp25 family protein	-
LVJ79_RS03520 (LVJ79_03520)	648794..649690	+	897	WP_003785643.1	baseplate J/gp47 family protein	-
LVJ79_RS03525 (LVJ79_03525)	649687..650268	+	582	WP_003785645.1	phage tail protein I	-
LVJ79_RS03530 (LVJ79_03530)	650286..651686	+	1401	WP_111694425.1	phage tail protein	-
LVJ79_RS03535 (LVJ79_03535)	651699..651908	+	210	WP_032133698.1	hypothetical protein	-
LVJ79_RS03540 (LVJ79_03540)	651853..652209	+	357	WP_003789002.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	637503..667263	29760

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-88)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-77)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10329.98 Da
• Isoelectric Point: 7.2429

>T233466 WP_003785639.1 NZ_CP091518:647429-647698 [Kingella kingae]
MSREIIFQGQFKRDVKKQFLELATVEWAEVLTCLVNDLPLAEKYRDHALVNDKNGYRDCHIKPDLVLIYGRKENALILVR
LGSHSEIFG

Nucleotide

• Length: 270 bp

>T233466 NZ_HG941718:325684-326604 [Escherichia coli O25b:H4-ST131]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10648.21 Da
• Isoelectric Point: 7.0134

>AT233466 WP_003785638.1 NZ_CP091518:647148-647432 [Kingella kingae]
MSAINFNIRMDKSLKERAFPVIESYGLTPAQAVKLFFNQIATTQTIPISFEHNVNRIPNAITRKAIEDAESEFETAKRYK
TVEEAIAAMQELAK

Nucleotide

• Length: 285 bp

>AT233466 NZ_HG941718:324915-325697 [Escherichia coli O25b:H4-ST131]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: F5S5C3

Antitoxin

• AlphaFold DB: F5S5C2