Detailed information of TA system TA233465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 379903..380549
• Replicon: chromosome
• Accession: NZ_CP091518
• Organism: Kingella kingae strain 4177/66

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: F5S4I4
• Locus tag: LVJ79_RS02110
• Protein ID: WP_003785163.1
• Coordinates: 380142..380549 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: F5S4I3
• Locus tag: LVJ79_RS02105
• Protein ID: WP_003785161.1
• Coordinates: 379903..380145 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ79_RS02085 (LVJ79_02085)	375300..376043	+	744	WP_003785156.1	IS110 family transposase	-
LVJ79_RS02090 (LVJ79_02090)	376308..376391	+	84	Protein_389	IS5/IS1182 family transposase	-
LVJ79_RS02095 (LVJ79_02095)	376827..377636	-	810	WP_003785159.1	hypothetical protein	-
LVJ79_RS02100 (LVJ79_02100)	377723..379762	-	2040	WP_003785160.1	excinuclease ABC subunit UvrB	-
LVJ79_RS02105 (LVJ79_02105)	379903..380145	+	243	WP_003785161.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LVJ79_RS02110 (LVJ79_02110)	380142..380549	+	408	WP_003785163.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ79_RS02115 (LVJ79_02115)	380609..381106	-	498	WP_003785166.1	thiol peroxidase	-
LVJ79_RS02120 (LVJ79_02120)	381200..381700	-	501	WP_003785168.1	hypothetical protein	-
LVJ79_RS02125 (LVJ79_02125)	381791..382969	-	1179	WP_003785170.1	phosphoglycerate kinase	-
LVJ79_RS02130 (LVJ79_02130)	383049..383681	-	633	WP_003791386.1	pyridoxamine 5'-phosphate oxidase	-
LVJ79_RS02135 (LVJ79_02135)	383901..385151	+	1251	WP_003785176.1	glutamyl-tRNA reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-125)

Antitoxin

• SpoVT-AbrB domain (6-40)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15241.45 Da
• Isoelectric Point: 6.8791

>T233465 WP_003785163.1 NZ_CP091518:380142-380549 [Kingella kingae]
MMYLLDTNILIYIQKNNPPTVREKINNLPNSAQLVMSFVTYAELLKGTYGSQNLEKAQANLNALTRIISVLPSHEAMPEH
YANWANQLKKQGKPIGNNDLWIAAHALAVGAVLVTHNTKEFSRINNLQLEDWVEE

Nucleotide

• Length: 408 bp

>T233465 NZ_HG941718:56173-56457 [Escherichia coli O25b:H4-ST131]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9242.32 Da
• Isoelectric Point: 4.1964

>AT233465 WP_003785161.1 NZ_CP091518:379903-380145 [Kingella kingae]
MLTKVFQSGNSQAVRIPMNFRFDVDTVEIFRTENGDVVLRSPKPQNRGDELFAILQQFDDDFIATVEARDNEPPQEREVL

Nucleotide

• Length: 243 bp

>AT233465 NZ_HG941718:55937-56170 [Escherichia coli O25b:H4-ST131]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: F5S4I4

Antitoxin

• AlphaFold DB: F5S4I3