Detailed information of TA system TA233459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 664..1250
• Replicon: plasmid p2Vster
• Accession: NZ_CP091514
• Organism: Vitreoscilla stercoraria strain SAG 1488-6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LVJ81_RS13035
• Protein ID: WP_019959540.1
• Coordinates: 664..918 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LVJ81_RS13040
• Protein ID: WP_019959539.1
• Coordinates: 915..1250 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ81_RS13030 (LVJ81_13030)	16..576	+	561	WP_026353726.1	recombinase family protein	-
LVJ81_RS13035 (LVJ81_13035)	664..918	+	255	WP_019959540.1	type II toxin-antitoxin system HicA family toxin	Toxin
LVJ81_RS13040 (LVJ81_13040)	915..1250	+	336	WP_019959539.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LVJ81_RS13045 (LVJ81_13045)	1607..3280	+	1674	WP_019959538.1	2-isopropylmalate synthase	-
LVJ81_RS13050 (LVJ81_13050)	3281..4042	-	762	WP_244802750.1	helix-turn-helix transcriptional regulator	-
LVJ81_RS13055 (LVJ81_13055)	4263..4457	+	195	WP_019959536.1	hypothetical protein	-
LVJ81_RS13060 (LVJ81_13060)	4884..5348	-	465	WP_019959535.1	hypothetical protein	-
LVJ81_RS13065 (LVJ81_13065)	5767..6054	-	288	WP_019959534.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12828	12828

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-75)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9570.05 Da
• Isoelectric Point: 10.6504

>T233459 WP_019959540.1 NZ_CP091514:664-918 [Vitreoscilla stercoraria]
MKRKHQKTLELIFHRPVSGSIKWTEIEALFIELGANVQEREGSRIAVVLFGQVKVFHRPHPSPDTDKGAVASVRKWLDEN
GVRP

Nucleotide

• Length: 255 bp

>T233459 NZ_HG938371:1169309-1169716 [Burkholderia cenocepacia H111]
ATGAAGTTCCTCCTGGATACCAACGCGGTGATCGCCATTCTCAAAGGCGAGCCGGCGATACTGGCCCGTTTGCATGCCTG
GCACCCGGCCGATTTCGGGATCCCCGCGATCGTCGCGCACGAGCTTTACTACGGCGCGTACAAGAGCCAGCGAGCGGCTG
CGAACGTCGCGCGTGTCGACGCGCTGCAGTTCGAGGTCGTATCGTTCGACACCGAGGATGCACAGCATGCGGGTGAAATT
CGTGCGCACCTGATCGCGGCCGGCACGCCGATCGGTCCATACGACGCCTTGATCGCAGGGCAGGCGCGTGCGCGGCATCT
GGTGCTGGTCACGCACAACGTCCGCGAATTCGAGCGCGTTCCGCGGCTGCAATTCGAAGACTGGCTCGCCGAACCGAGCG
CAGACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12199.91 Da
• Isoelectric Point: 5.6459

>AT233459 WP_019959539.1 NZ_CP091514:915-1250 [Vitreoscilla stercoraria]
MINTMIINGYKAVIAYDSEIELFRGEFIGLNGGADFYAADVESLRREGAESLKVFLEVCKENGIEPKKQYSGRFNVRINP
KLHEDAMAAAIAQGISLNELVSRAIATAAHH

Nucleotide

• Length: 336 bp

>AT233459 NZ_HG938371:1169070-1169312 [Burkholderia cenocepacia H111]
ATGGATACTGCAAAGCTCTTCAAGCATGGCGGCTCGCAGGCTGTCCGGCTACCGAAGGATTTCCGCTTCGATACGGCCGA
GGTTCGCATCCGCCGCCACGGGGCGGCCGTCATTCTCGAGCCGATGCCGCAAGACTGGGCTTGGCTGGCACCGCTGATCG
GTCCGGTGGACGCCGATTTCGAGGCTGCCGCGACCACACAGCCGTCCGCTCAGGAGCGCTCGGGCCTGGACGTGTTCGAA
TGA