Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 36977..37502 Replicon plasmid unnamed3
Accession NZ_CP091499
Organism Enterobacter cloacae complex sp. ECL404

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag L3071_RS25835 Protein ID WP_008322213.1
Coordinates 36977..37282 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag L3071_RS25840 Protein ID WP_032934863.1
Coordinates 37284..37502 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3071_RS25805 (L3071_25805) 32115..32231 + 117 Protein_38 IS5 family transposase -
L3071_RS25810 (L3071_25810) 32303..33423 + 1121 WP_089046468.1 IS3-like element ISSen4 family transposase -
L3071_RS25815 (L3071_25815) 33451..34197 + 747 Protein_40 IS5 family transposase -
L3071_RS25820 (L3071_25820) 34359..35681 + 1323 WP_016479957.1 GntP family transporter -
L3071_RS25825 (L3071_25825) 35880..36671 - 792 WP_008322208.1 site-specific integrase -
L3071_RS25830 (L3071_25830) 36661..36975 - 315 WP_008322211.1 hypothetical protein -
L3071_RS25835 (L3071_25835) 36977..37282 - 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
L3071_RS25840 (L3071_25840) 37284..37502 - 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L3071_RS25845 (L3071_25845) 38362..39059 - 698 Protein_46 IS1 family transposase -
L3071_RS25850 (L3071_25850) 39272..41854 + 2583 WP_016479955.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 htpB 1..99480 99480
- inside IScluster/Tn - - 26766..54100 27334


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T233450 WP_008322213.1 NZ_CP091499:c37282-36977 [Enterobacter cloacae complex sp. ECL404]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T233450 NZ_HG938356:c108507-108253 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGAAGAAACGCCATCGCGCCACGCTTGAGCTGATCTATGCAAGGCCCGCGAGCGGTTCGATCCGATGGGTGGATATCGA
GGCCATGTTCGTAGCGCTTGGTGCGGACGTGACCGAGCGGGAGGGATCCCGGATCGGCGTGGTGCTGTTCGGCGAGGTCA
GGGTATTTCATCGCCCACATCCATCGCCCGATACGGACAAAGGCGCGGTCGCCAGCATTCGAAAATGGCTCGAAAGCCAT
GGAGTGAAACCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT233450 WP_032934863.1 NZ_CP091499:c37502-37284 [Enterobacter cloacae complex sp. ECL404]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT233450 NZ_HG938356:c108256-107921 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGAGGAACGTCATCGAAATCGAAGGCCATAAAGCGGTTGTCTCACTTGATCCGGAGATCGGCATGTTTCGCGGGGAGTT
CCTCGGCCTGAATGGCGGCGCCGACTTCTACTCCGACAGTCTCGATGGCCTGATGTCGGAGGGTCAAAAGTCGCTCCAGA
CCTTCCTTGAAATCTGCAAGGAAAAGGGGATTGAGCCTTTTCGCTCGTTTTCGGGGCGATTTAACGTCCGGCTCGATCCG
CGCACCCATGAAGCGGCCGTCATCGCCGCTACCGCCAACGATCAAAGCCTCAACGAATGGCTTGCCGAGACAATCGCGAC
CGCAGCACGGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References