Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | kacAT/ElaA-DUF1778 |
| Location | 27914..28717 | Replicon | plasmid unnamed3 |
| Accession | NZ_CP091499 | ||
| Organism | Enterobacter cloacae complex sp. ECL404 | ||
Toxin (Protein)
| Gene name | KacT | Uniprot ID | W8E895 |
| Locus tag | L3071_RS25785 | Protein ID | WP_016479963.1 |
| Coordinates | 27914..28444 (-) | Length | 177 a.a. |
Antitoxin (Protein)
| Gene name | KacA | Uniprot ID | W8E6Q5 |
| Locus tag | L3071_RS25790 | Protein ID | WP_022652312.1 |
| Coordinates | 28448..28717 (-) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L3071_RS25755 (L3071_25755) | 23129..23419 | + | 291 | WP_004201172.1 | co-chaperone GroES | - |
| L3071_RS25760 (L3071_25760) | 23475..25115 | + | 1641 | WP_004201176.1 | chaperonin GroEL | - |
| L3071_RS25765 (L3071_25765) | 25304..25486 | + | 183 | WP_016479964.1 | hypothetical protein | - |
| L3071_RS25770 (L3071_25770) | 25741..25860 | + | 120 | Protein_31 | IS30 family transposase | - |
| L3071_RS25775 (L3071_25775) | 25873..26619 | + | 747 | Protein_32 | IS3 family transposase | - |
| L3071_RS25780 (L3071_25780) | 26766..27746 | - | 981 | WP_000019403.1 | IS5-like element IS5 family transposase | - |
| L3071_RS25785 (L3071_25785) | 27914..28444 | - | 531 | WP_016479963.1 | GNAT family N-acetyltransferase | Toxin |
| L3071_RS25790 (L3071_25790) | 28448..28717 | - | 270 | WP_022652312.1 | DUF1778 domain-containing protein | Antitoxin |
| L3071_RS25795 (L3071_25795) | 29573..30562 | + | 990 | WP_022652311.1 | RepB family plasmid replication initiator protein | - |
| L3071_RS25800 (L3071_25800) | 30858..31597 | - | 740 | Protein_37 | site-specific integrase | - |
| L3071_RS25805 (L3071_25805) | 32115..32231 | + | 117 | Protein_38 | IS5 family transposase | - |
| L3071_RS25810 (L3071_25810) | 32303..33423 | + | 1121 | WP_089046468.1 | IS3-like element ISSen4 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaNDM-1 | htpB | 1..99480 | 99480 | |
| - | inside | IScluster/Tn | - | - | 26766..54100 | 27334 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 177 a.a. Molecular weight: 20350.24 Da Isoelectric Point: 6.4907
>T233449 WP_016479963.1 NZ_CP091499:c28444-27914 [Enterobacter cloacae complex sp. ECL404]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE
Download Length: 531 bp
>T233449 NZ_HG938356:c101989-101474 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGCCTGAATTGGCCCCGGAATCAGGCATCCTCTGGCGTGCCTATGTGCCGCGCTGGGCGCATGCGCCGCTTTCCGGCGA
CGGCGCTGCCCGTTTTGGCGGACGCTGGAACCCGGTTGGCGCAGCGACCATCTATGCGGCGCGCGAGCTCTCTACCGCCT
GGGCGGAGTATAATCAGGGTTTCGTCCAGCATCCGGCCCTGATCGCCCAGCTTCGGCTCGACGGCGCGCGGCTCGCCGAT
CTGACCAGCCCTGAGGTTCTGTCCGGTCTCGGTGTCGACGAGACCATCCACCGCTGCGAATGGCGAGCCGATCTCGATGC
CGGCCGGATACCGGCGACGCATCTCCTCGCAGAGAGGTTGCTCGATGATGGCCGGGACGGCGTCATCTATCCGTCATTCA
TGTCGCCGGGCGGAACCTGCGTCGCCCTCTGGCGCTGGAACGGCAAGGACCAGCCGAAACTCACGGTCACCGACCCCGAC
GGGCGGTTGCCGAAAAACCCGGCTTCCTGGTTGTAG
ATGCCTGAATTGGCCCCGGAATCAGGCATCCTCTGGCGTGCCTATGTGCCGCGCTGGGCGCATGCGCCGCTTTCCGGCGA
CGGCGCTGCCCGTTTTGGCGGACGCTGGAACCCGGTTGGCGCAGCGACCATCTATGCGGCGCGCGAGCTCTCTACCGCCT
GGGCGGAGTATAATCAGGGTTTCGTCCAGCATCCGGCCCTGATCGCCCAGCTTCGGCTCGACGGCGCGCGGCTCGCCGAT
CTGACCAGCCCTGAGGTTCTGTCCGGTCTCGGTGTCGACGAGACCATCCACCGCTGCGAATGGCGAGCCGATCTCGATGC
CGGCCGGATACCGGCGACGCATCTCCTCGCAGAGAGGTTGCTCGATGATGGCCGGGACGGCGTCATCTATCCGTCATTCA
TGTCGCCGGGCGGAACCTGCGTCGCCCTCTGGCGCTGGAACGGCAAGGACCAGCCGAAACTCACGGTCACCGACCCCGAC
GGGCGGTTGCCGAAAAACCCGGCTTCCTGGTTGTAG
Antitoxin
Download Length: 90 a.a. Molecular weight: 10269.70 Da Isoelectric Point: 5.7252
>AT233449 WP_022652312.1 NZ_CP091499:c28717-28448 [Enterobacter cloacae complex sp. ECL404]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE
Download Length: 270 bp
>AT233449 NZ_HG938356:c102350-101982 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGGCTCATGCTTTTGAAATCGGCGCCGCCGCTTTCCGTGAACAGCAATCCGCGCTTCTGTCGGCGCGGCGGGTGTCCGA
TCTGCTCGGACTGACCCAGGCGGAACTTGCGAAACTGATCGGTGTCGCCCGCAACACGCTGACGGCCAAAAGCGGCGCCC
GCAAGGTCGATCTGGCGCTCTCCCCGGTCGTGCGCATCCTGGCGATGGCGAGCGAGATGGCCGGCGACGAGAACCGCGCG
GCGATCTGGTTCAAGCACCAGCCTATCCCGGGTTGGGCCGGCAAGACGGCTTACGACCTCGTCGGCGAAGGCAAGGCCGA
CAAGGTGCTCGCCTATCTGGAGGCGGTGCGCTCCGGCGTTTATGCCTGA
ATGGCTCATGCTTTTGAAATCGGCGCCGCCGCTTTCCGTGAACAGCAATCCGCGCTTCTGTCGGCGCGGCGGGTGTCCGA
TCTGCTCGGACTGACCCAGGCGGAACTTGCGAAACTGATCGGTGTCGCCCGCAACACGCTGACGGCCAAAAGCGGCGCCC
GCAAGGTCGATCTGGCGCTCTCCCCGGTCGTGCGCATCCTGGCGATGGCGAGCGAGATGGCCGGCGACGAGAACCGCGCG
GCGATCTGGTTCAAGCACCAGCCTATCCCGGGTTGGGCCGGCAAGACGGCTTACGACCTCGTCGGCGAAGGCAAGGCCGA
CAAGGTGCTCGCCTATCTGGAGGCGGTGCGCTCCGGCGTTTATGCCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6C0L7Q2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z3XDS5 |