Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 88142..88667 Replicon plasmid unnamed2
Accession NZ_CP091498
Organism Enterobacter cloacae complex sp. ECL404

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag L3071_RS25050 Protein ID WP_008322213.1
Coordinates 88362..88667 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag L3071_RS25045 Protein ID WP_032934863.1
Coordinates 88142..88360 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3071_RS25030 (84291) 84291..84722 + 432 WP_000790483.1 silver-binding protein SilE -
L3071_RS25035 (84866) 84866..85216 + 351 WP_000694954.1 DUF305 domain-containing protein -
L3071_RS25040 (85383) 85383..87209 - 1827 WP_127851252.1 AAA family ATPase -
L3071_RS25045 (88142) 88142..88360 + 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L3071_RS25050 (88362) 88362..88667 + 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
L3071_RS25055 (88669) 88669..88981 + 313 Protein_97 cytoplasmic protein -
L3071_RS25060 (88971) 88971..89760 + 790 Protein_98 site-specific integrase -
L3071_RS25065 (89959) 89959..91279 - 1321 Protein_99 GntP family transporter -
L3071_RS25070 (91441) 91441..92187 - 747 Protein_100 IS5 family transposase -
L3071_RS25080 (93408) 93408..93524 - 117 Protein_102 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / mph(A) - 1..181169 181169


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T233448 WP_008322213.1 NZ_CP091498:88362-88667 [Enterobacter cloacae complex sp. ECL404]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T233448 NZ_HG938356:c43909-43619 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGATCGAAGTCCGCAAAACGGCAGCTTTCTCAAAGTGGTTCGGCGAACTTCGCGACCATAACGCCCGAATGCGGATAGT
TACGCGGATTCGCCGTATGGAGATCGGCAATCTGGGGGATGTAAAACCTGTCGGCGAAGGCATCAGCGAAGCCCGTATCG
CCTACGGCCCCGGCTACCGGCTCTATTTCATACAGCAAGGCCAGGAGATCATCATTCTGCTTTGCGGCGGTGACAAGAGC
ACCCAGAGCAAGGACATCGCTTCAGCAAAACAGATGGCGAAGGAGATATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT233448 WP_032934863.1 NZ_CP091498:88142-88360 [Enterobacter cloacae complex sp. ECL404]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT233448 NZ_HG938356:c43616-43320 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
GTGCCTATTGAAACGACGAAATGGGACGTTCAGGACTACCTGAAGACGCCCGAAGAGCGCGTTGCCTATCTCGAAGCGGC
TTTCGAAGATGGCGATCCAAAGCTTATCTCGATCGCCCTTGGCGATGTTGCCCGCTCCATGGGCATGACCACCGTCGCCA
AGGAAGCGGGCATTACCCGCGAGGCGCTCTACAAGGCGCTGAGCGATAAGGGAGATCCGAAGCTCTCCACACTGCTCGGC
GTCTTGAAGGCCCTCGGGCTCCGGGTCACGGTCACTTCCGCGAACGAAGCGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References