Detailed information of TA system TA233447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 32236..32975
• Replicon: plasmid unnamed2
• Accession: NZ_CP091498
• Organism: Enterobacter cloacae complex sp. ECL404

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: L3071_RS24740
• Protein ID: WP_127851260.1
• Coordinates: 32236..32721 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: L3071_RS24745
• Protein ID: WP_127851259.1
• Coordinates: 32709..32975 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3071_RS24710 (27435)	27435..27686	+	252	WP_236904355.1	universal stress protein	-
L3071_RS24715 (27746)	27746..28171	-	426	WP_000065802.1	glutaredoxin-dependent arsenate reductase	-
L3071_RS24720 (28184)	28184..29473	-	1290	WP_000922628.1	arsenic transporter	-
L3071_RS24725 (29519)	29519..29837	-	319	Protein_31	metalloregulator ArsR/SmtB family transcription factor	-
L3071_RS24730 (29922)	29922..30625	+	704	Protein_32	arsenical resistance protein ArsH	-
L3071_RS24735 (30658)	30658..32061	-	1404	WP_000125668.1	ISNCY family transposase	-
L3071_RS24740 (32236)	32236..32721	-	486	WP_127851260.1	GNAT family N-acetyltransferase	Toxin
L3071_RS24745 (32709)	32709..32975	-	267	WP_127851259.1	DUF1778 domain-containing protein	Antitoxin
L3071_RS24750 (33597)	33597..34610	+	1014	WP_148577035.1	plasmid replication initiator RepA	-
L3071_RS24755 (35455)	35455..35727	+	273	WP_217189052.1	DUF2767 family protein	-
L3071_RS24760 (35909)	35909..36913	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
L3071_RS24765 (37389)	37389..37760	+	372	WP_127851293.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / mph(A)	-	1..181169	181169
	-	inside	IScluster/Tn	-	-	30658..36913	6255

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17721.55 Da
• Isoelectric Point: 10.1380

>T233447 WP_127851260.1 NZ_CP091498:c32721-32236 [Enterobacter cloacae complex sp. ECL404]
VGRVTAPEPLSSFHQLAEFVSGEVVLDDWLKQRGLKNQALGAARTFVVCKKNTKLVAGFYSLATGSVNHAEATGSLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEARSFYLHHGFKLSQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T233447 NZ_HG938356:c43616-43320 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
GTGCCTATTGAAACGACGAAATGGGACGTTCAGGACTACCTGAAGACGCCCGAAGAGCGCGTTGCCTATCTCGAAGCGGC
TTTCGAAGATGGCGATCCAAAGCTTATCTCGATCGCCCTTGGCGATGTTGCCCGCTCCATGGGCATGACCACCGTCGCCA
AGGAAGCGGGCATTACCCGCGAGGCGCTCTACAAGGCGCTGAGCGATAAGGGAGATCCGAAGCTCTCCACACTGCTCGGC
GTCTTGAAGGCCCTCGGGCTCCGGGTCACGGTCACTTCCGCGAACGAAGCGGCATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10065.45 Da
• Isoelectric Point: 4.4733

>AT233447 WP_127851259.1 NZ_CP091498:c32975-32709 [Enterobacter cloacae complex sp. ECL404]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVSNDAAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT233447 NZ_HG938356:c43909-43619 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGATCGAAGTCCGCAAAACGGCAGCTTTCTCAAAGTGGTTCGGCGAACTTCGCGACCATAACGCCCGAATGCGGATAGT
TACGCGGATTCGCCGTATGGAGATCGGCAATCTGGGGGATGTAAAACCTGTCGGCGAAGGCATCAGCGAAGCCCGTATCG
CCTACGGCCCCGGCTACCGGCTCTATTTCATACAGCAAGGCCAGGAGATCATCATTCTGCTTTGCGGCGGTGACAAGAGC
ACCCAGAGCAAGGACATCGCTTCAGCAAAACAGATGGCGAAGGAGATATGA