Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hipBA/HipA-HipB |
| Location | 79938..81561 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP091497 | ||
| Organism | Enterobacter cloacae complex sp. ECL404 | ||
Toxin (Protein)
| Gene name | hipA | Uniprot ID | C1IUI3 |
| Locus tag | L3071_RS23185 | Protein ID | WP_000088044.1 |
| Coordinates | 80236..81561 (+) | Length | 442 a.a. |
Antitoxin (Protein)
| Gene name | hipB | Uniprot ID | A0A6M3PBR7 |
| Locus tag | L3071_RS23180 | Protein ID | WP_012477377.1 |
| Coordinates | 79938..80231 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L3071_RS23160 (L3071_23160) | 75737..76129 | + | 393 | WP_000341066.1 | hypothetical protein | - |
| L3071_RS23165 (L3071_23165) | 76788..77444 | + | 657 | WP_000462754.1 | trypsin-like serine protease | - |
| L3071_RS23170 (L3071_23170) | 77647..78144 | + | 498 | WP_000340139.1 | membrane protein | - |
| L3071_RS23175 (L3071_23175) | 78149..79537 | + | 1389 | WP_012695445.1 | hypothetical protein | - |
| L3071_RS23180 (L3071_23180) | 79938..80231 | + | 294 | WP_012477377.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| L3071_RS23185 (L3071_23185) | 80236..81561 | + | 1326 | WP_000088044.1 | type II toxin-antitoxin system HipA family toxin | Toxin |
| L3071_RS23190 (L3071_23190) | 81622..81828 | + | 207 | WP_000134171.1 | hypothetical protein | - |
| L3071_RS23195 (L3071_23195) | 81929..82339 | + | 411 | WP_000985909.1 | hypothetical protein | - |
| L3071_RS23200 (L3071_23200) | 82352..82876 | + | 525 | WP_001805097.1 | hypothetical protein | - |
| L3071_RS23205 (L3071_23205) | 83058..83672 | - | 615 | Protein_81 | IS110-like element IS4321 family transposase | - |
| L3071_RS23210 (L3071_23210) | 83728..84696 | - | 969 | WP_072202995.1 | IS5 family transposase | - |
| L3071_RS23215 (L3071_23215) | 84729..85127 | - | 399 | Protein_83 | IS110 family transposase | - |
| L3071_RS23220 (L3071_23220) | 85218..85652 | - | 435 | WP_000405672.1 | Cd(II)/Pb(II)-responsive transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaTEM-1B / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / aph(3')-Ia / tet(D) / catA2 / blaSHV-12 / sul1 / blaDHA-1 / qnrB4 / qacE / ere(A) / aac(6')-IIc | - | 1..319000 | 319000 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 442 a.a. Molecular weight: 50333.32 Da Isoelectric Point: 6.5044
>T233445 WP_000088044.1 NZ_CP091497:80236-81561 [Enterobacter cloacae complex sp. ECL404]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
Download Length: 1326 bp
>T233445 NZ_HG938355:c4495402-4495115 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGGCTGAAAAACTAACCGTTTACGATCCAGCCGAAGACCTGGGCTCCGATCAGGCGATTGCGGCATTTATGGCCGAAGC
TTTCGAGACGGAGGACGCGGCCTACATTGCGCATGCCTTAGGGGTGGTTGCCCGTGCCAAGGGGATGATGCAGATTGCCA
AAGACACCGGTCTTTCCCGAGAGCAGCTCTATCGTTCCTTTAGCGCCACCGGAAACCCGACGCTCAAGACGACGATTGCA
GTGATGAAATCGTTGGGGATCGAGTTGACCGCCAAGGCCCATTCCTGA
ATGGCTGAAAAACTAACCGTTTACGATCCAGCCGAAGACCTGGGCTCCGATCAGGCGATTGCGGCATTTATGGCCGAAGC
TTTCGAGACGGAGGACGCGGCCTACATTGCGCATGCCTTAGGGGTGGTTGCCCGTGCCAAGGGGATGATGCAGATTGCCA
AAGACACCGGTCTTTCCCGAGAGCAGCTCTATCGTTCCTTTAGCGCCACCGGAAACCCGACGCTCAAGACGACGATTGCA
GTGATGAAATCGTTGGGGATCGAGTTGACCGCCAAGGCCCATTCCTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 10860.30 Da Isoelectric Point: 10.3134
>AT233445 WP_012477377.1 NZ_CP091497:79938-80231 [Enterobacter cloacae complex sp. ECL404]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW
Download Length: 294 bp
>AT233445 NZ_HG938355:c4495698-4495402 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
GTGTTCGAGCTCAAGCAGACGGAAACGTTTCTGAAATGGCGCACGCGTCTGAAGGACGAGCGTGCTCGCGCATTGATAGC
CTCGCGTCTGGACCGGCTCGCTTTCGGGCATGCCGGAGATGTGGCCCCCGTCGGCGAAGGTGTCAGCGAACTGCGCATCC
ATCATGGGCCGGGTTACCGCGTCTATTTTCAGCAGCGCGGTGACATCCTAATCGTTCTTCTGTGCGGTGGCGACAAAAGC
AGCCAGGCAAAGGACATCAAGGCTGCAAAGCGACTGGCGATGGAATGGAGTGAGTGA
GTGTTCGAGCTCAAGCAGACGGAAACGTTTCTGAAATGGCGCACGCGTCTGAAGGACGAGCGTGCTCGCGCATTGATAGC
CTCGCGTCTGGACCGGCTCGCTTTCGGGCATGCCGGAGATGTGGCCCCCGTCGGCGAAGGTGTCAGCGAACTGCGCATCC
ATCATGGGCCGGGTTACCGCGTCTATTTTCAGCAGCGCGGTGACATCCTAATCGTTCTTCTGTGCGGTGGCGACAAAAGC
AGCCAGGCAAAGGACATCAAGGCTGCAAAGCGACTGGCGATGGAATGGAGTGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2S1XXW1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6M3PBR7 |