Detailed information of TA system TA233444

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4310749..4311406
• Replicon: chromosome
• Accession: NZ_CP091496
• Organism: Enterobacter cloacae complex sp. ECL404

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: L3071_RS20495
• Protein ID: WP_038416677.1
• Coordinates: 4310996..4311406 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: G8LDB7
• Locus tag: L3071_RS20490
• Protein ID: WP_003863437.1
• Coordinates: 4310749..4311015 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3071_RS20470 (L3071_20470)	4306181..4307614	-	1434	WP_038416681.1	6-phospho-beta-glucosidase BglA	-
L3071_RS20475 (L3071_20475)	4307731..4308462	-	732	WP_022649308.1	MurR/RpiR family transcriptional regulator	-
L3071_RS20480 (L3071_20480)	4308729..4309388	+	660	WP_022649307.1	hemolysin III family protein	-
L3071_RS20485 (L3071_20485)	4309474..4310454	-	981	WP_038416679.1	tRNA-modifying protein YgfZ	-
L3071_RS20490 (L3071_20490)	4310749..4311015	+	267	WP_003863437.1	FAD assembly factor SdhE	Antitoxin
L3071_RS20495 (L3071_20495)	4310996..4311406	+	411	WP_038416677.1	protein YgfX	Toxin
L3071_RS20500 (L3071_20500)	4311408..4311929	-	522	WP_023306886.1	flavodoxin FldB	-
L3071_RS20505 (L3071_20505)	4312031..4312927	+	897	WP_003863442.1	site-specific tyrosine recombinase XerD	-
L3071_RS20510 (L3071_20510)	4312956..4313669	+	714	WP_022649304.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
L3071_RS20515 (L3071_20515)	4313675..4315408	+	1734	WP_038416675.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16337.20 Da
• Isoelectric Point: 11.4775

>T233444 WP_038416677.1 NZ_CP091496:4310996-4311406 [Enterobacter cloacae complex sp. ECL404]
VVLWQSDLRVSWRSQWMSLLLHGLVAAMVLLVPWPLSYTPLWLLLLSFVVFDSVRSQRRINSRQGEIKLLMDSRLRWQGK
EWEMIGTPWMLNTGMMLRLRRVEDNRRQHLWLAADSMDPAEWRDLRRLIVQQPTQE

Nucleotide

• Length: 411 bp

>T233444 NZ_HG938355:4391334-4391642 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGAACCTGATGCGTTTCGAATGGGATCGCGCAAAGGCGGCGACGAACGTCAAGAAGCACGGCATCAGCTTCGAGACCGC
GGCGCGTGTTTTCGCCGACCCTTTCGTCCTGATCGAGCAGGATCGTATCGAAGGTGGAGAGCAGCGTTGGCAGGCACTGG
GTTCTGTTGATGGTCTCGCCATCCTGCTCGTTGCTCACACCGTCTACGAGGACGAGGAGGATGACGGCCAGGCCTACGAG
GTGATCCGCATCATTTCGGCCCGAAAAGCCGATCCGAGAGAAAGACGACGTTATGAAGAAGAGCTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10526.96 Da
• Isoelectric Point: 4.8310

>AT233444 WP_003863437.1 NZ_CP091496:4310749-4311015 [Enterobacter cloacae complex sp. ECL404]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
QERGPVAI

Nucleotide

• Length: 267 bp

>AT233444 NZ_HG938355:4391626-4391955 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGAAGAAGAGCTCTGAGATCGTCAGGTACGAGGTCGATAGGGATAGTCTGCCGCCTCTGACCGAAAAGCAGAGGGCTGA
GCTTGCAGCCCTGTCGAAACTTCCTGACGAGCAGATAGATTACAGCGACATTCCAGGCTTGACCGACGAACAACTCCAGA
ATGCCGGGCGGGGACGATTTTATCGCCCCCTGAAACAGCAGATCACGGCCCGGGTTGATGCCGATGTTGTGGACTGGCTC
AAGTCGCAGGGCAAAGGTTATCAGGCCCGCATGAATGCAATCCTTCGCCGGGAAATGCTGGCGTCGCTGAAATCCCAGAA
GCGGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A837F8P5