Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3487147..3487763 Replicon chromosome
Accession NZ_CP091496
Organism Enterobacter cloacae complex sp. ECL404

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag L3071_RS16555 Protein ID WP_080298797.1
Coordinates 3487392..3487763 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A9E7BIL4
Locus tag L3071_RS16550 Protein ID WP_023298920.1
Coordinates 3487147..3487389 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3071_RS16520 (L3071_16520) 3482205..3483005 + 801 WP_038416949.1 DeoR/GlpR family DNA-binding transcription regulator -
L3071_RS16525 (L3071_16525) 3483124..3483723 + 600 WP_023315056.1 glucose-1-phosphatase -
L3071_RS16530 (L3071_16530) 3483717..3484598 + 882 WP_023298923.1 virulence factor BrkB family protein -
L3071_RS16535 (L3071_16535) 3484595..3485032 + 438 WP_015569909.1 D-aminoacyl-tRNA deacylase -
L3071_RS16540 (L3071_16540) 3485077..3486018 + 942 WP_022649838.1 fatty acid biosynthesis protein FabY -
L3071_RS16545 (L3071_16545) 3486027..3486947 - 921 WP_038416944.1 alpha/beta hydrolase -
L3071_RS16550 (L3071_16550) 3487147..3487389 + 243 WP_023298920.1 ribbon-helix-helix protein, CopG family Antitoxin
L3071_RS16555 (L3071_16555) 3487392..3487763 + 372 WP_080298797.1 type II toxin-antitoxin system VapC family toxin Toxin
L3071_RS16560 (L3071_16560) 3487803..3488732 - 930 WP_022649835.1 formate dehydrogenase accessory protein FdhE -
L3071_RS16565 (L3071_16565) 3488729..3489364 - 636 WP_006808711.1 formate dehydrogenase cytochrome b556 subunit -
L3071_RS16570 (L3071_16570) 3489361..3490263 - 903 WP_003861960.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-114)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-49)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13612.66 Da        Isoelectric Point: 6.4865

>T233443 WP_080298797.1 NZ_CP091496:3487392-3487763 [Enterobacter cloacae complex sp. ECL404]
MEHMAVYDTNILIDLFNNHVEAADAIDRTASHRAISLITWMEVMVGARRHGQEAKTAAVMGAFEIIDITREIAERSVLLR
EQHGMKLPDAIILATAQSRSCPLISRNTKDFSGIAGVVSPYHL

Download         Length: 372 bp

>T233443 NZ_HG938355:c3676754-3676464 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGAAAACTCTGATCTTCGCGCCGAAGGCGACCGCGGACATCGACCATATTTACGATTATACAGACGAGACCTGGGGCTA
CAATCAGGCTGAAGAATATACGTTCGGAATCCGGGATTTTTGTAGGGCACTTTCCTTGGGGGAGAGAAGCGGACGGAAGA
TCGACCAGATCAAACGCGGATATCGAGCGCTTGCCTACGGGTCCCACTTTATCGTCTATCGAGAAACGCCTACGACAATC
TCGATCATCCGCGTCCTTCACCAGCGCATGAATATCGGCGGCCATCTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9467.74 Da        Isoelectric Point: 4.3775

>AT233443 WP_023298920.1 NZ_CP091496:3487147-3487389 [Enterobacter cloacae complex sp. ECL404]
MSLLADLTMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIELYLDQQNSSVIREALGLWGNQQEDGLEYERKLREEW

Download         Length: 243 bp

>AT233443 NZ_HG938355:c3676999-3676751 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGGGCAAGATGACATCGATCACAGTGGATGAGGAACTGACTGAGTTCATCGACAGGCAGGTGAGCGGCGGCAACTACAG
TTCGCCAAGCGAAGTGGTGGAGGCCGCCTTACGGCTTCTGCGTGACAGCGAGGGCGGTATCGAGGCCATTCGCGCGGCGA
TCGAAGAAGGCGAGGCCTCGGGCGAGCCGCAGCCTTTCGATTTCGACGAGTTCATTGAAAAGAAACGTGCCTTGCGCGCC
GAGCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References