Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2305814..2306435 Replicon chromosome
Accession NZ_CP091496
Organism Enterobacter cloacae complex sp. ECL404

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag L3071_RS10950 Protein ID WP_015571250.1
Coordinates 2306217..2306435 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag L3071_RS10945 Protein ID WP_006809850.1
Coordinates 2305814..2306188 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3071_RS10935 (L3071_10935) 2300942..2302135 + 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L3071_RS10940 (L3071_10940) 2302158..2305304 + 3147 WP_023299464.1 multidrug efflux RND transporter permease subunit AcrB -
L3071_RS10945 (L3071_10945) 2305814..2306188 + 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
L3071_RS10950 (L3071_10950) 2306217..2306435 + 219 WP_015571250.1 HHA domain-containing protein Toxin
L3071_RS10955 (L3071_10955) 2306643..2307194 + 552 WP_022647270.1 maltose O-acetyltransferase -
L3071_RS10960 (L3071_10960) 2307311..2307778 + 468 WP_022647269.1 YlaC family protein -
L3071_RS10965 (L3071_10965) 2307750..2309210 - 1461 WP_038415532.1 PLP-dependent aminotransferase family protein -
L3071_RS10970 (L3071_10970) 2309313..2310023 + 711 WP_038415530.1 GNAT family protein -
L3071_RS10975 (L3071_10975) 2310020..2310160 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
L3071_RS10980 (L3071_10980) 2310163..2310423 - 261 WP_015571255.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T233442 WP_015571250.1 NZ_CP091496:2306217-2306435 [Enterobacter cloacae complex sp. ECL404]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T233442 NZ_HG938355:c2725817-2725539 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGATCAAATCTTGGGTTAATAGTTCAACCCGTCGCTTTGCCGAGGAAGGCAAATCCAAGTTTTCGGGGATGGACGAAGA
GGCCGCGATCGAGTTGTTGGCGGCGCTCCACGCGGCCTCGTCCCTGAACGATCTCAGTCCATTGAAATCGGTTGGCCTTC
ACAAGCTTTCCGGAAATCGCTCAGGACAGTGGGCAATGACGATCAACGGGCCGTGGCGGATCTGTTTCACGTTCAGGGAT
GGAGATGCCTGGGACGTGGAAATCGTCGACTATCATTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT233442 WP_006809850.1 NZ_CP091496:2305814-2306188 [Enterobacter cloacae complex sp. ECL404]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT233442 NZ_HG938355:c2725525-2725241 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGATAGCAGTTCATCCGGGTCGCATTTTGAAGCGCGAACTCACCGCACGCAGCCTGTCGGCCAATCAGCTCGCTCTTTC
ACTTCGCCTGCCGTCAGGCCGCATCACCGACATTCTCAATGCAAAGCGCGGCATCTCGCCCGATACAGCCCTGAGATTGG
CGCGGTATTTTGGAAACAGTGCCCGCTTTTGGCTTGATCTGCAGACCGCATATGAACTCGCTCTTGCCGAACGAGAAAAT
GGCGAACGGATCGCGGAAGAGGTCAAACCGGCCGAAGCTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References