Detailed information of TA system TA233441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1091504..1092243
• Replicon: chromosome
• Accession: NZ_CP091496
• Organism: Enterobacter cloacae complex sp. ECL404

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: L3071_RS05105
• Protein ID: WP_038416064.1
• Coordinates: 1091758..1092243 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: L3071_RS05100
• Protein ID: WP_003857131.1
• Coordinates: 1091504..1091770 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3071_RS05075 (L3071_05075)	1086598..1087176	+	579	WP_022648146.1	fimbrial protein	-
L3071_RS05080 (L3071_05080)	1087200..1088336	+	1137	WP_038416067.1	type 1 fimbrial protein	-
L3071_RS05085 (L3071_05085)	1088457..1089014	+	558	WP_023300131.1	OmpH family outer membrane protein	-
L3071_RS05090 (L3071_05090)	1088993..1090381	-	1389	WP_038416066.1	MFS transporter	-
L3071_RS05095 (L3071_05095)	1090511..1091440	+	930	WP_038416065.1	LysR family transcriptional regulator	-
L3071_RS05100 (L3071_05100)	1091504..1091770	+	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
L3071_RS05105 (L3071_05105)	1091758..1092243	+	486	WP_038416064.1	GNAT family N-acetyltransferase	Toxin
L3071_RS05110 (L3071_05110)	1092704..1094107	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
L3071_RS05115 (L3071_05115)	1094136..1094768	-	633	WP_001567369.1	hypothetical protein	-
L3071_RS05120 (L3071_05120)	1094843..1095214	+	372	Protein_1008	IS5/IS1182 family transposase	-
L3071_RS05125 (L3071_05125)	1095246..1096213	+	968	Protein_1009	IS5-like element IS903B family transposase	-
L3071_RS05130 (L3071_05130)	1096374..1097188	+	815	Protein_1010	APH(3')-I family aminoglycoside O-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17546.28 Da
• Isoelectric Point: 9.9308

>T233441 WP_038416064.1 NZ_CP091496:1091758-1092243 [Enterobacter cloacae complex sp. ECL404]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALAAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGKGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T233441 NZ_HG938355:c2518423-2518154 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGAAGTTCGAATTCGATCCTGACAAGAGCGCCACGAACAAGGATAAGCACGGAATCGATTTCGTGGAGGCGCAAGCGCT
ATGGGCGGATGAGAGGCGGACCGTCGCTCCGGTCCAGTCGGATACGGAGTCGCGATATATCCTTACCGGAAAGATTGCGG
AGAAGCATTGGACCGCGGTCTATACATGGCGAGGAGACTGGCTGCGAATTATATCGGTCAGGCGCGCACGTGACAAAGAG
AAGCAGACCTATGAAGACAATAACCGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT233441 WP_003857131.1 NZ_CP091496:1091504-1091770 [Enterobacter cloacae complex sp. ECL404]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT233441 NZ_HG938355:c2518173-2517931 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGAAGACAATAACCGCTGAAGAGTTTGACAAGAAATTCGACGATGGCGAGGACATCAGCGAATATGTGGACTGGTCTAA
GGCCACACGCCCTGGTCTCGAGCCCAAGCGCGTCAATATCGATTTTCCGACCTGGGTCGTGAACGGGCTGGATCAGGAGG
CGCGGCGTCTTGGTGTTACGCGGCAGTCGCTGGTGAAACTCTGGATCGCCGAACGGCTTGAGGCAAGACGCCAAGGCAAG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9