Detailed information of TA system TA023344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3667368..3668168
• Replicon: chromosome
• Accession: NC_011741
• Organism: Escherichia coli IAI1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: ECIAI1_RS18015
• Protein ID: WP_000342449.1
• Coordinates: 3667368..3667895 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: ECIAI1_RS18020
• Protein ID: WP_001277108.1
• Coordinates: 3667902..3668168 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECIAI1_RS17990	3662443..3663210	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
ECIAI1_RS17995	3663207..3664484	-	1278	WP_000803784.1	branched chain amino acid ABC transporter permease LivM	-
ECIAI1_RS18000	3664481..3665407	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
ECIAI1_RS18005	3665455..3666564	-	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
ECIAI1_RS18010	3666988..3667371	+	384	WP_000778785.1	aspartate 1-decarboxylase autocleavage activator PanM	-
ECIAI1_RS18015	3667368..3667895	-	528	WP_000342449.1	GNAT family N-acetyltransferase	Toxin
ECIAI1_RS18020	3667902..3668168	-	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
ECIAI1_RS18025	3668318..3669421	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
ECIAI1_RS18030	3669692..3670546	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
ECIAI1_RS18035	3670791..3671849	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
ECIAI1_RS18040	3671842..3672510	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T23344 WP_000342449.1 NC_011741:c3667895-3667368 [Escherichia coli IAI1]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T23344 NC_011741:c3667895-3667368 [Escherichia coli IAI1]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT23344 WP_001277108.1 NC_011741:c3668168-3667902 [Escherichia coli IAI1]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT23344 NC_011741:c3668168-3667902 [Escherichia coli IAI1]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A829CLZ4

Antitoxin

• AlphaFold DB: A0A829CN24