Detailed information of TA system TA233434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 57978..58504
• Replicon: plasmid unnamed2
• Accession: NZ_CP091494
• Organism: Enterobacter cloacae complex sp. ECL405

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7R6HXL1
• Locus tag: L3070_RS25805
• Protein ID: WP_015572079.1
• Coordinates: 57978..58265 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: L3070_RS25810
• Protein ID: WP_000534858.1
• Coordinates: 58265..58504 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3070_RS25780 (L3070_25780)	53117..54121	+	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
L3070_RS25785 (L3070_25785)	54401..55018	-	618	Protein_54	plasmid replication initiator RepA	-
L3070_RS25790 (L3070_25790)	55599..56387	-	789	WP_015572118.1	DsbA family protein	-
L3070_RS25795 (L3070_25795)	56416..57024	-	609	WP_094935623.1	hypothetical protein	-
L3070_RS25800 (L3070_25800)	57752..57925	-	174	WP_229231590.1	DUF5431 family protein	-
L3070_RS25805 (L3070_25805)	57978..58265	-	288	WP_015572079.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L3070_RS25810 (L3070_25810)	58265..58504	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
L3070_RS25815 (L3070_25815)	58529..58628	+	100	Protein_60	hypothetical protein	-
L3070_RS25820 (L3070_25820)	58679..59578	-	900	WP_045325182.1	LysR family transcriptional regulator	-
L3070_RS25825 (L3070_25825)	59671..60156	+	486	WP_045325184.1	cyclophilin-like fold protein	-
L3070_RS25830 (L3070_25830)	60284..61501	+	1218	WP_072206003.1	MFS transporter	-
L3070_RS25835 (L3070_25835)	61518..62564	+	1047	WP_045325187.1	NAD(P)-dependent alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..160020	160020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11209.09 Da
• Isoelectric Point: 10.0714

>T233434 WP_015572079.1 NZ_CP091494:c58265-57978 [Enterobacter cloacae complex sp. ECL405]
MAYFLDFDERALKEWRKLGSTVREQLKNKLAEVLESPRIDANKLRGMHDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T233434 NZ_HG938355:670242-670487 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGAAGGCTGTACTCTATTCTAAGGTCGCGCAAAAAAGCCTGAGCCGGATGCAGCCCAAACGCCAGGCCGCCATCAGGGC
GAAGGTCGATGCGTTCGCCCGTGGCGACAGAGTGGATCTCAAAAGGCTGGAGGGTAGCGCGCTCGTTCGCATCCGTGTCG
GCAATGACAGGATCATCATCGATGAGGAGACGAACCTCGTCGTTGTTATCGATGTCGGGCCCAGAGGCGGTATCTATAAG
GAGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT233434 WP_000534858.1 NZ_CP091494:c58504-58265 [Enterobacter cloacae complex sp. ECL405]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT233434 NZ_HG938355:670491-670844 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGGGTGTTTTTGACAAGACCGTCATCGACGGCAAGTCGTATGTCCTGGTTCCCGAAGACGACTTCGAGGATATGATGGA
CACCATCAAAGCCAATGAAATCCTGGCGCGCATCGCCGCCGGTGAGGAAACATGGCCTGCCGAGCTGGTGTACGAACTGC
TTGAAACCGAAAGCCGCATCCGTACTTTCCGCAACTACCGCAAGATGACGGTCTCGGAGCTTGCGGAAGCAGCCGGCATT
TCTCAACCCTACCTGTCCGAGATCGAGACCGGCAAGAAGACAGGCTCGGTCGATGTGCTGAAGCGCATCGCCGCCGTCCT
GAAAGTCGATCTCGACGATCTCGTGCTGGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A4V1CTS8