Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 57978..58504 Replicon plasmid unnamed2
Accession NZ_CP091494
Organism Enterobacter cloacae complex sp. ECL405

Toxin (Protein)


Gene name relE Uniprot ID A0A7R6HXL1
Locus tag L3070_RS25805 Protein ID WP_015572079.1
Coordinates 57978..58265 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag L3070_RS25810 Protein ID WP_000534858.1
Coordinates 58265..58504 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3070_RS25780 (L3070_25780) 53117..54121 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
L3070_RS25785 (L3070_25785) 54401..55018 - 618 Protein_54 plasmid replication initiator RepA -
L3070_RS25790 (L3070_25790) 55599..56387 - 789 WP_015572118.1 DsbA family protein -
L3070_RS25795 (L3070_25795) 56416..57024 - 609 WP_094935623.1 hypothetical protein -
L3070_RS25800 (L3070_25800) 57752..57925 - 174 WP_229231590.1 DUF5431 family protein -
L3070_RS25805 (L3070_25805) 57978..58265 - 288 WP_015572079.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L3070_RS25810 (L3070_25810) 58265..58504 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
L3070_RS25815 (L3070_25815) 58529..58628 + 100 Protein_60 hypothetical protein -
L3070_RS25820 (L3070_25820) 58679..59578 - 900 WP_045325182.1 LysR family transcriptional regulator -
L3070_RS25825 (L3070_25825) 59671..60156 + 486 WP_045325184.1 cyclophilin-like fold protein -
L3070_RS25830 (L3070_25830) 60284..61501 + 1218 WP_072206003.1 MFS transporter -
L3070_RS25835 (L3070_25835) 61518..62564 + 1047 WP_045325187.1 NAD(P)-dependent alcohol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..160020 160020


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.09 Da        Isoelectric Point: 10.0714

>T233434 WP_015572079.1 NZ_CP091494:c58265-57978 [Enterobacter cloacae complex sp. ECL405]
MAYFLDFDERALKEWRKLGSTVREQLKNKLAEVLESPRIDANKLRGMHDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T233434 NZ_HG938355:670242-670487 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGAAGGCTGTACTCTATTCTAAGGTCGCGCAAAAAAGCCTGAGCCGGATGCAGCCCAAACGCCAGGCCGCCATCAGGGC
GAAGGTCGATGCGTTCGCCCGTGGCGACAGAGTGGATCTCAAAAGGCTGGAGGGTAGCGCGCTCGTTCGCATCCGTGTCG
GCAATGACAGGATCATCATCGATGAGGAGACGAACCTCGTCGTTGTTATCGATGTCGGGCCCAGAGGCGGTATCTATAAG
GAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT233434 WP_000534858.1 NZ_CP091494:c58504-58265 [Enterobacter cloacae complex sp. ECL405]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT233434 NZ_HG938355:670491-670844 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
ATGGGTGTTTTTGACAAGACCGTCATCGACGGCAAGTCGTATGTCCTGGTTCCCGAAGACGACTTCGAGGATATGATGGA
CACCATCAAAGCCAATGAAATCCTGGCGCGCATCGCCGCCGGTGAGGAAACATGGCCTGCCGAGCTGGTGTACGAACTGC
TTGAAACCGAAAGCCGCATCCGTACTTTCCGCAACTACCGCAAGATGACGGTCTCGGAGCTTGCGGAAGCAGCCGGCATT
TCTCAACCCTACCTGTCCGAGATCGAGACCGGCAAGAAGACAGGCTCGGTCGATGTGCTGAAGCGCATCGCCGCCGTCCT
GAAAGTCGATCTCGACGATCTCGTGCTGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References