Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1057016..1057636 Replicon chromosome
Accession NZ_CP091492
Organism Enterobacter cloacae complex sp. ECL405

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag L3070_RS04970 Protein ID WP_008499287.1
Coordinates 1057016..1057234 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag L3070_RS04975 Protein ID WP_008499288.1
Coordinates 1057262..1057636 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3070_RS04940 (L3070_04940) 1053030..1053290 + 261 WP_045356432.1 type B 50S ribosomal protein L31 -
L3070_RS04945 (L3070_04945) 1053293..1053433 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
L3070_RS04950 (L3070_04950) 1053430..1054140 - 711 WP_087822105.1 GNAT family protein -
L3070_RS04955 (L3070_04955) 1054242..1055702 + 1461 WP_094934746.1 PLP-dependent aminotransferase family protein -
L3070_RS04960 (L3070_04960) 1055674..1056141 - 468 WP_045400719.1 YlaC family protein -
L3070_RS04965 (L3070_04965) 1056259..1056810 - 552 WP_047061252.1 maltose O-acetyltransferase -
L3070_RS04970 (L3070_04970) 1057016..1057234 - 219 WP_008499287.1 HHA domain-containing protein Toxin
L3070_RS04975 (L3070_04975) 1057262..1057636 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
L3070_RS04980 (L3070_04980) 1058148..1061294 - 3147 WP_029741964.1 multidrug efflux RND transporter permease subunit AcrB -
L3070_RS04985 (L3070_04985) 1061317..1062510 - 1194 WP_029741963.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T233415 WP_008499287.1 NZ_CP091492:c1057234-1057016 [Enterobacter cloacae complex sp. ECL405]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T233415 NZ_HG938353:c340978-340709 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
TTGGCTTGGACGATTGAGCTTCGGGCGAGCGCGGAAAAGCAATTGGGCCGCCTCGCAAAACGGGATGCTGCGCGCATCAT
TTCCTTTCTTGAAGAACGCCTTGCGACCCACGAAAACCCCAGAGAGTTGGGCGACGCGCTGAAAGGACACGCCCTCGGCG
GATATTGGCGTTACCGCGTCGGCGACTACCGGATCATCTGCGACATCCAGGACCAGCGACTTGTCGTGCTGGTGATCGAG
ATCGGCCATCGCCGCGAGGTCTATCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT233415 WP_008499288.1 NZ_CP091492:c1057636-1057262 [Enterobacter cloacae complex sp. ECL405]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT233415 NZ_HG938353:c341192-340962 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
ATGAACAAGCGCGTCACGATCGAAATGCCGGAAGAGATGCATGAGGCGATTACAGAGCATGCGGCCAAGGCCGGTGCTAA
AACCGACGCGTATGTTCTGGATCTGATCGAACAGCATCTGGAAGACCTCCACGACATCGCACTCGCCGAAGCCGCCATGG
AGCGCATTCGCGATGGTGAGCCGACCTATACTCTGGAAGAAGTGAAGCTCCGCCTTGGCTTGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References