Detailed information of TA system TA233414

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 932205..932920
• Replicon: chromosome
• Accession: NZ_CP091492
• Organism: Enterobacter cloacae complex sp. ECL405

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8I0UM05
• Locus tag: L3070_RS04380
• Protein ID: WP_023303149.1
• Coordinates: 932552..932920 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8I0UJV2
• Locus tag: L3070_RS04375
• Protein ID: WP_023303148.1
• Coordinates: 932205..932531 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3070_RS04350 (L3070_04350)	927233..928498	+	1266	WP_023303143.1	hypothetical protein	-
L3070_RS04355 (L3070_04355)	928961..929242	+	282	WP_126802433.1	hypothetical protein	-
L3070_RS04360 (L3070_04360)	929301..930140	+	840	WP_023303145.1	hypothetical protein	-
L3070_RS26975	930652..930774	-	123	WP_255740893.1	hypothetical protein	-
L3070_RS04365 (L3070_04365)	930830..931651	+	822	WP_063612981.1	DUF932 domain-containing protein	-
L3070_RS04370 (L3070_04370)	931722..932192	+	471	WP_063612980.1	DNA repair protein RadC	-
L3070_RS04375 (L3070_04375)	932205..932531	+	327	WP_023303148.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L3070_RS04380 (L3070_04380)	932552..932920	+	369	WP_023303149.1	TA system toxin CbtA family protein	Toxin
L3070_RS04385 (L3070_04385)	933135..933812	-	678	WP_112778430.1	type A chloramphenicol O-acetyltransferase	-
L3070_RS04390 (L3070_04390)	933876..935807	-	1932	WP_047061211.1	bifunctional diguanylate cyclase/phosphodiesterase	-
L3070_RS04395 (L3070_04395)	935825..936319	-	495	WP_039263874.1	molybdopterin-dependent oxidoreductase	-
L3070_RS04400 (L3070_04400)	936719..937306	-	588	WP_094935827.1	cytochrome b	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	911281..936319	25038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13727.91 Da
• Isoelectric Point: 8.2822

>T233414 WP_023303149.1 NZ_CP091492:932552-932920 [Enterobacter cloacae complex sp. ECL405]
MKNLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIILADAVNFLVDKYELVRIDRRGF
SSQGQVPYLTVTDILHARRACGLMKSCSYREVSNIVLSHSRQ

Nucleotide

• Length: 369 bp

>T233414 NZ_HG917973:4835-5212 [Mycobacterium marinum E11]
GTGACCGAATACCCCACAGTCGATGATGTGCTGATCGTCGCAGAGTATGCCTGCGGCTTCCGTCCTCTGGTCAGCGACCC
AGGTCTATTCGAGTCGAGTCTGATGCGCCCAGCGGCAACAGTTTTCGGTAGCGACGCCTACCCGACTGTGTGGGATAAGG
CGGCAGCGCTGCTGCATTCGTTCGTGACGACGCAGTCGCTGGCCGATGGAAACAAGCGGACAGGCTGGGCTGCGTGCTGG
CTTCTACTTGGCCTCAATGGGCACCGGCTATCTGCGGCCCTTGACGTCGATGCGGCCGAGAAGTTCGTTCTTGCTATCGC
TGGCGGCGAACTAGCTTGGGAACAGATCGCAGCTCGACTGCCCGATTTCGCAGATTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12215.04 Da
• Isoelectric Point: 7.0266

>AT233414 WP_023303148.1 NZ_CP091492:932205-932531 [Enterobacter cloacae complex sp. ECL405]
MSLIPAHEWGIKSDIVPRLGVRLVQEGNRLHYLADRASITGKFSDAECLKLDIVFPHFIRQMESMLTTGEMNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp

>AT233414 NZ_HG917973:4641-4838 [Mycobacterium marinum E11]
ATGCCGGCGTTGAATGTCGATTTTTCCGACGAGGAACTGGCGATCGTGCGGTCGGCGGCGAGCAACGCCGGCGCGTCGAT
GCGTGGCTTTGCAAAGCAAGCGATCCTGGACAAGGCAGTTGACCGGGCCGGGCGAGTGCACGCCCTCGGGCGAGAGATCG
CGGATCGGTCGGCTGAACTGAATCGCCGGTTAGCGTGA