Detailed information of TA system TA233413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 594521..595097
• Replicon: chromosome
• Accession: NZ_CP091492
• Organism: Enterobacter cloacae complex sp. ECL405

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A4R0G892
• Locus tag: L3070_RS02780
• Protein ID: WP_010427219.1
• Coordinates: 594521..594808 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A411GC49
• Locus tag: L3070_RS02785
• Protein ID: WP_033144548.1
• Coordinates: 594795..595097 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3070_RS02755 (L3070_02755)	589621..590316	+	696	WP_094935517.1	winged helix-turn-helix domain-containing protein	-
L3070_RS02760 (L3070_02760)	590467..590937	+	471	WP_094935516.1	MarR family transcriptional regulator	-
L3070_RS02765 (L3070_02765)	590934..592001	+	1068	WP_094935515.1	HlyD family secretion protein	-
L3070_RS02770 (L3070_02770)	592006..593082	+	1077	WP_023310256.1	DUF2955 domain-containing protein	-
L3070_RS02775 (L3070_02775)	593079..594350	-	1272	WP_094935514.1	DUF445 domain-containing protein	-
L3070_RS02780 (L3070_02780)	594521..594808	+	288	WP_010427219.1	BrnT family toxin	Toxin
L3070_RS02785 (L3070_02785)	594795..595097	+	303	WP_033144548.1	BrnA antitoxin family protein	Antitoxin
L3070_RS02790 (L3070_02790)	595128..595766	-	639	WP_094935513.1	LysE family translocator	-
L3070_RS02795 (L3070_02795)	595814..596557	-	744	WP_048976422.1	AraC family transcriptional regulator	-
L3070_RS02800 (L3070_02800)	596709..598079	+	1371	WP_094935512.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
L3070_RS02805 (L3070_02805)	598126..598446	-	321	WP_039262264.1	hypothetical protein	-
L3070_RS02810 (L3070_02810)	598446..598991	-	546	WP_023310269.1	YfaZ family outer membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11174.64 Da
• Isoelectric Point: 6.7609

>T233413 WP_010427219.1 NZ_CP091492:594521-594808 [Enterobacter cloacae complex sp. ECL405]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T233413 NZ_HG917972:c5367549-5367103 [Mycobacterium marinum E11]
ATGGCGAAACTTTCCGGGTCGATCGACGTGCCGCTGTCTCCGGACGAGGCATGGATGCACGCATCCGATCTCTCCCGCTT
CAAGGAGTGGCTGACGATCCACCGAGTGTGGCGCAGCACGTTGCCCGAAACCCTCGAAAAGGGTGCGGTCGTCGAATCAA
TCGTGCAGGTCAAGGGCATGCACAACCGCATCAAATGGACCATCGTGCGCTACCAGCCGCCGGAAGGCATGACCCTCAAC
GGTGACGGGGTCGGCGGCGTCAAGGTCAAGCTGATGGCCAAGGTTGCAGCCGGAGAAGAAGGCTCGGTCGTCAGCTTCGA
CGTGCACCTTGGTGGGCCGGCTCTGTTCGGTCCGATCGGCATGGTCGTCGCCGCGGCGCTGCGCACCGATATCAACGCAT
CGCTGCGGAACTTCGTCACCGTATTCGCCCCGTCGGCCGCCGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11395.04 Da
• Isoelectric Point: 10.2483

>AT233413 WP_033144548.1 NZ_CP091492:594795-595097 [Enterobacter cloacae complex sp. ECL405]
MSMVKHKRGSAPEMSARREAELKALAEKSEAEIDYSDIPATGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT233413 NZ_HG917972:c5367742-5367554 [Mycobacterium marinum E11]
ATGGGAATCCTGGACAAGGCGAAAGACCTGCTGGCGCAGAACGCCGACAAGGTCGAGACGGTGATCGACAAGGCCGGCGA
ATTCGTCGACGAGAAGACCGAGGGGAAGTACTCCGACACCATCCACAAGGTGCAAGAGGGGGCCAAGAAAGTCGCTGGTC
CCGGCGCCGCGGGCGAGCAGCAGAACTGA

Structures

Toxin

• AlphaFold DB: A0A4R0G892

Antitoxin

• AlphaFold DB: A0A411GC49