Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 33440..34243 Replicon plasmid unnamed5
Accession NZ_CP091491
Organism Enterobacter cloacae complex sp. ECL411

Toxin (Protein)


Gene name KacT Uniprot ID W8E895
Locus tag L3069_RS26965 Protein ID WP_016479963.1
Coordinates 33713..34243 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag L3069_RS26960 Protein ID WP_022652312.1
Coordinates 33440..33709 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3069_RS26945 (L3069_26945) 29926..30042 - 117 Protein_27 IS5 family transposase -
L3069_RS26950 (L3069_26950) 30560..31299 + 740 Protein_28 site-specific integrase -
L3069_RS26955 (L3069_26955) 31595..32584 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
L3069_RS26960 (L3069_26960) 33440..33709 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
L3069_RS26965 (L3069_26965) 33713..34243 + 531 WP_016479963.1 GNAT family N-acetyltransferase Toxin
L3069_RS26970 (L3069_26970) 34411..35391 + 981 WP_000019403.1 IS5-like element IS5 family transposase -
L3069_RS26975 (L3069_26975) 35538..36284 - 747 Protein_33 IS3 family transposase -
L3069_RS26980 (L3069_26980) 36297..36416 - 120 Protein_34 IS30 family transposase -
L3069_RS26985 (L3069_26985) 36671..36853 - 183 WP_016479964.1 hypothetical protein -
L3069_RS26990 (L3069_26990) 37042..38682 - 1641 WP_004201176.1 chaperonin GroEL -
L3069_RS26995 (L3069_26995) 38738..39028 - 291 WP_004201172.1 co-chaperone GroES -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 htpB 1..99483 99483
- inside IScluster/Tn - - 8054..36203 28149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20350.24 Da        Isoelectric Point: 6.4907

>T233412 WP_016479963.1 NZ_CP091491:33713-34243 [Enterobacter cloacae complex sp. ECL411]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T233412 NZ_HG917972:3435857-3436189 [Mycobacterium marinum E11]
GTGAATAGAGGGGAGATCTGGACCGTAGCGGGGGGCGTCTACGCGACCAAACCGCATCCCGCGGTGATTGTTCAGGATGA
TCTCTTTGATGCCACGAGTTCGGTGACGGTTGCTCCGATGTCCAGCACCCTGTTGGATGCGCCGTTGATGCGCATCCGAA
TAGCCGGCGGAGACGGCCGGTTATCCGGACTTGATCACAACAGTGACGTGATGATCGACAAGCTCACAACCGTGAAAAGG
TCGAACGTCCATGCCCGAGTCGGTCGGCTGACAGCGGAGCAAGTCGTCGAGGTCGAGCGAGTGATGATGGCATTCCTCGG
CCTTGCGCGATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT233412 WP_022652312.1 NZ_CP091491:33440-33709 [Enterobacter cloacae complex sp. ECL411]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT233412 NZ_HG917972:3435654-3435860 [Mycobacterium marinum E11]
ATGGCGGTTAGGGACAGGGTCGGCGAGTACCGACGGCGGATGCGGGAGCGGGGCCTGCGGCCGCTGCAGGTCTGGGTGCC
TGACGTGCGCACTGAGAGTTTTGCTGCCGAGGCGCATCGTCAGGCTTCGTTGGTCGCACGAGCGGACGAACGCGGCGATG
ATCAGGACTTCATTGAGGCCATCTCGACGCCATGGGATGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0L7Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References