Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 24654..25179 Replicon plasmid unnamed5
Accession NZ_CP091491
Organism Enterobacter cloacae complex sp. ECL411

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag L3069_RS26915 Protein ID WP_008322213.1
Coordinates 24874..25179 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag L3069_RS26910 Protein ID WP_032934863.1
Coordinates 24654..24872 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3069_RS26900 (L3069_26900) 20300..22882 - 2583 WP_016479955.1 EAL domain-containing protein -
L3069_RS26905 (L3069_26905) 23095..23792 + 698 Protein_19 IS1 family transposase -
L3069_RS26910 (L3069_26910) 24654..24872 + 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L3069_RS26915 (L3069_26915) 24874..25179 + 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
L3069_RS26920 (L3069_26920) 25181..25495 + 315 WP_008322211.1 hypothetical protein -
L3069_RS26925 (L3069_26925) 25485..26276 + 792 WP_008322208.1 site-specific integrase -
L3069_RS26930 (L3069_26930) 26475..27797 - 1323 WP_016479957.1 GntP family transporter -
L3069_RS26935 (L3069_26935) 27959..28705 - 747 Protein_25 IS5 family transposase -
L3069_RS26945 (L3069_26945) 29926..30042 - 117 Protein_27 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 htpB 1..99483 99483
- inside IScluster/Tn - - 8054..36203 28149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T233411 WP_008322213.1 NZ_CP091491:24874-25179 [Enterobacter cloacae complex sp. ECL411]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T233411 NZ_HG916826:c4517327-4517076 [Pseudomonas pseudoalcaligenes CECT 5344]
ATGGACGCCATCACCTACAGCACCGCACGCGCCAACCTGGCCAATACCATGGATCGCGTATGCGAGGATCACGAACCCAT
CATCATCACCCGCAATGGTCAGCAGGCTGTGGTGATGATGTCGCTCGACGACTACAAGGCACTGGAAGAAACCGCCTACC
TGCTGCGTAGCCCTAAGAACGCACAGCGTCTGCTCGAAAGTATCGCCGCGCTTGAGACGGGCAAGGGAGTCACGCGAGAG
CTTGACCTGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT233411 WP_032934863.1 NZ_CP091491:24654-24872 [Enterobacter cloacae complex sp. ECL411]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT233411 NZ_HG916826:c4517079-4516825 [Pseudomonas pseudoalcaligenes CECT 5344]
GTGAATCTGGTGTTCTCCGAGCAAGCCTGGGCGGATTACCTCTACTGGCAAGGGCAGGACAAGAAGACCCTGAAACGGAT
CAACGAACTGCTCAAGGAAATCAAAAGAACGCCATTCGAGGGCATGGGCAAACCCGAACCGCTCAAGCACGCCCTGGCGG
GCTACTGGTCGCGGCGGATCAACGAAGAGCATCGTCTGGTGTACAGGCTCCGAGAGCATGAAATTCTGATCGCCCAGGTG
CGTTATCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References