Detailed information of TA system TA233410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 79486..80283
• Replicon: plasmid unnamed4
• Accession: NZ_CP091490
• Organism: Enterobacter cloacae complex sp. ECL411

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: L3069_RS26665
• Protein ID: WP_172754447.1
• Coordinates: 79486..79989 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: L3069_RS26670
• Protein ID: WP_016582626.1
• Coordinates: 80014..80283 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3069_RS26640 (L3069_26640)	77156..77944	+	789	WP_040110304.1	receptor-recognizing protein	-
L3069_RS26645 (L3069_26645)	78019..78342	+	324	WP_000064173.1	hypothetical protein	-
L3069_RS26650 (L3069_26650)	78356..79048	+	693	WP_006812500.1	membrane protein	-
L3069_RS26655 (L3069_26655)	79050..79301	+	252	WP_040110303.1	hypothetical protein	-
L3069_RS26660 (L3069_26660)	79294..79458	+	165	WP_228100666.1	hypothetical protein	-
L3069_RS26665 (L3069_26665)	79486..79989	-	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
L3069_RS26670 (L3069_26670)	80014..80283	-	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
L3069_RS26675 (L3069_26675)	80700..81365	+	666	WP_023315962.1	AAA family ATPase	-
L3069_RS26680 (L3069_26680)	81371..81724	+	354	WP_160956455.1	hypothetical protein	-
L3069_RS26685 (L3069_26685)	81766..82563	-	798	WP_077990606.1	DUF2971 domain-containing protein	-
L3069_RS26690 (L3069_26690)	82827..83552	+	726	WP_006812583.1	hypothetical protein	-
L3069_RS26695 (L3069_26695)	83613..84953	+	1341	WP_006812582.1	DnaB-like helicase C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..107172	107172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T233410 WP_172754447.1 NZ_CP091490:c79989-79486 [Enterobacter cloacae complex sp. ECL411]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T233410 NZ_HG916826:c4517079-4516825 [Pseudomonas pseudoalcaligenes CECT 5344]
GTGAATCTGGTGTTCTCCGAGCAAGCCTGGGCGGATTACCTCTACTGGCAAGGGCAGGACAAGAAGACCCTGAAACGGAT
CAACGAACTGCTCAAGGAAATCAAAAGAACGCCATTCGAGGGCATGGGCAAACCCGAACCGCTCAAGCACGCCCTGGCGG
GCTACTGGTCGCGGCGGATCAACGAAGAGCATCGTCTGGTGTACAGGCTCCGAGAGCATGAAATTCTGATCGCCCAGGTG
CGTTATCACTACTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT233410 WP_016582626.1 NZ_CP091490:c80283-80014 [Enterobacter cloacae complex sp. ECL411]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT233410 NZ_HG916826:c4517327-4517076 [Pseudomonas pseudoalcaligenes CECT 5344]
ATGGACGCCATCACCTACAGCACCGCACGCGCCAACCTGGCCAATACCATGGATCGCGTATGCGAGGATCACGAACCCAT
CATCATCACCCGCAATGGTCAGCAGGCTGTGGTGATGATGTCGCTCGACGACTACAAGGCACTGGAAGAAACCGCCTACC
TGCTGCGTAGCCCTAAGAACGCACAGCGTCTGCTCGAAAGTATCGCCGCGCTTGAGACGGGCAAGGGAGTCACGCGAGAG
CTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3