Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 44443..44968 Replicon plasmid unnamed2
Accession NZ_CP091488
Organism Enterobacter cloacae complex sp. ECL411

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag L3069_RS24785 Protein ID WP_008322213.1
Coordinates 44663..44968 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag L3069_RS24780 Protein ID WP_032934863.1
Coordinates 44443..44661 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3069_RS24765 (40592) 40592..41023 + 432 WP_000790483.1 silver-binding protein SilE -
L3069_RS24770 (41167) 41167..41517 + 351 WP_000694954.1 DUF305 domain-containing protein -
L3069_RS24775 (41684) 41684..43510 - 1827 WP_127851252.1 AAA family ATPase -
L3069_RS24780 (44443) 44443..44661 + 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L3069_RS24785 (44663) 44663..44968 + 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
L3069_RS24790 (44970) 44970..45284 + 315 WP_008322211.1 hypothetical protein -
L3069_RS24795 (45274) 45274..46065 + 792 WP_008322208.1 site-specific integrase -
L3069_RS24800 (46264) 46264..47586 - 1323 WP_016479957.1 GntP family transporter -
L3069_RS24805 (47748) 47748..48494 - 747 Protein_54 IS5 family transposase -
L3069_RS24815 (49715) 49715..49831 - 117 Protein_56 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) - 1..165214 165214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T233409 WP_008322213.1 NZ_CP091488:44663-44968 [Enterobacter cloacae complex sp. ECL411]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T233409 NZ_HG916826:c3556790-3556515 [Pseudomonas pseudoalcaligenes CECT 5344]
ATGTTGCCAATCATCTGGCGCGATAGCGCCAGAGCCGACTTGGCGGAAATCATCCGTTATATCGCTGATGAAGCCCTACA
GGCTGCCAGGCAGCTTAAGAATTACCTTGAATCTGCCGTCTTGCCGTTGGCTGAACACCCCTATCTGTATCGTTCCGGTC
GGGTTCCCGGCACTCGCGAGCTGGTCGCCCATCCCAACTATGTCTTGGTCTACAGGGTGGCGGCCGAGTGCATAGAAGTG
GTCAGCGTCCTTCATTCTCGCCAAGAATATCCTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT233409 WP_032934863.1 NZ_CP091488:44443-44661 [Enterobacter cloacae complex sp. ECL411]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT233409 NZ_HG916826:c3556984-3556790 [Pseudomonas pseudoalcaligenes CECT 5344]
ATGAGTGTGCAACTTTCCCCCATCGTGTCGGAGTTCGAGACGCAGGAGCAGGCGGATAGTCATGATCGCTGGTTTCGCCG
CAAGGTAAAGGAGTCTTTGGATGACTCGCGTCCTGGTGTTCCGCATGACCAAGTGATGGCAGAAATGGAAGCCATCATTG
TTCAGGCTGAAGCTCGGCAGCGAGGCCGTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References