Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 74895..76518 Replicon plasmid unnamed1
Accession NZ_CP091487
Organism Enterobacter cloacae complex sp. ECL411

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag L3069_RS23235 Protein ID WP_000088044.1
Coordinates 74895..76220 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag L3069_RS23240 Protein ID WP_012477377.1
Coordinates 76225..76518 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3069_RS23200 (L3069_23200) 70804..71238 + 435 WP_000405672.1 Cd(II)/Pb(II)-responsive transcriptional regulator -
L3069_RS23205 (L3069_23205) 71329..71727 + 399 Protein_86 IS110 family transposase -
L3069_RS23210 (L3069_23210) 71760..72728 + 969 WP_072202995.1 IS5 family transposase -
L3069_RS23215 (L3069_23215) 72784..73398 + 615 Protein_88 IS110-like element IS4321 family transposase -
L3069_RS23220 (L3069_23220) 73580..74104 - 525 WP_001805097.1 hypothetical protein -
L3069_RS23225 (L3069_23225) 74117..74527 - 411 WP_000985909.1 hypothetical protein -
L3069_RS23230 (L3069_23230) 74628..74834 - 207 WP_000134171.1 hypothetical protein -
L3069_RS23235 (L3069_23235) 74895..76220 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
L3069_RS23240 (L3069_23240) 76225..76518 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
L3069_RS23245 (L3069_23245) 76919..78307 - 1389 WP_012695445.1 hypothetical protein -
L3069_RS23250 (L3069_23250) 78312..78809 - 498 WP_000340139.1 membrane protein -
L3069_RS23255 (L3069_23255) 79012..79668 - 657 WP_000462754.1 trypsin-like serine protease -
L3069_RS23260 (L3069_23260) 80327..80719 - 393 WP_000341066.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / blaTEM-1B / aac(6')-IIc / ere(A) / qacE / sul1 / qnrB4 / blaDHA-1 / catA2 / tet(D) - 1..316592 316592


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T233407 WP_000088044.1 NZ_CP091487:c76220-74895 [Enterobacter cloacae complex sp. ECL411]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T233407 NZ_HG916826:1219466-1220035 [Pseudomonas pseudoalcaligenes CECT 5344]
ATGTTCAAGCCGCAGCTGGTGACTTTGCAACGTGGCGCTCTGCGCCTGGAACCGCTCTCTGACGCCGATATCCCCGCGCT
GGTGGCGCTGGCCGAAGCCAATCGCGAGGAGCTGCTGTACATGAGCGGTACTCAGCGACTGGACTGGTACCGCAGCGCCC
TGGCCGATATGCGCGAGCAGCGGGCACTGCCGTTCGTCATTCGTCTGGGTAACGAGTGGGTAGGCACCACCCGCTTCGCC
GACTTCATGAACCCCCTGCCGGCCTGTGAAATCGGCTGGACCTGGCTCGACCGCAATCAGCATGGCAGCGGGTTGAACAC
CAGCATCAAGTACCTGATGCTCAAGCACGCTTTCGAAAATTGGGGCATGGTTCGCGTGCAACTGAAAACTGCCGCCAGCA
ACCTGCGTTCGCAACGCGCCATCGAGAAGCTTGGTGCCGTTCGTGAAGGGTTGCTGCGCAACCATCGCCGTCTGGCTGAT
GGCCGACTCGATGACACCGTGCTGTACAGCATCACCGACCTGGAATGGCCGGCGCTGCGTGGGGCCATGGAGGCGGGCTT
CAACGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT233407 WP_012477377.1 NZ_CP091487:c76518-76225 [Enterobacter cloacae complex sp. ECL411]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT233407 NZ_HG916826:1220041-1220868 [Pseudomonas pseudoalcaligenes CECT 5344]
ATGCACAGTCGCGAGCAGACCCGCTTCTGGCAAGCTTCCGCATTGAGCGGCGTCGAGCTGTTGCAGGCGCGCTACCTTGA
ACAGCGCTTCGCGCCCCACGTGCATGAAGGTTTCGTGTTCACCGTGATCGAGCATGGCGCCCAGCGCTTTCGTCATCGCG
GTTGTGATCATCTGGCCCCACAGGGCAGCATGGTGCTGATCAACCCGGACGAAGTGCACACCGGCTCCAAGGCGCACGAA
GACGGTTGGCGCTATCGCGGTTTCTATCCCGACAACGCCCAGGTTCTGGGGGCGCTGCAGGAGCTGGGTCTGGCCAAGGC
TGGCATGCCGTCGTTTGCCTTCAGTGTGCTGCATGATCCACGTTTGCACGCGGCCTTTCTGCAACTGCACCAACTGCTCG
AGGGCGGCGCCGAGGCACTGCAGCAGCAACTGGCCTGGCGTGAGGCAATGCTGCTGCTGTTTCAGCACCATGCGCAGCTT
GCCGAACCACCTGCGCCTGGGCGTGAGCCCTGGGCAGTGGCTTGTGCCAAGCAGATGCTCGCCGAGCGGCTGATCGAGCC
GCCCTCGCTGGAGGAACTGGCGGCTGCGGTCAATCTTTCACCGTTTCATTTCGCTCGAGTGTTCCGCCGCGAGACCGGCT
TGCCACCGCATGCCTGGCTCAAGCAACGGCGCCTGGAGCAGGCGCGGGCGTTGCTCAAGACGGGCTGCACGGCGGTCGCT
GTGGCCATGCAGTTGGGCTTCGCTGATCAGAGCCACTTGTCGCGACAGTTCAAGCAGGTTTATGGCGTGAGCCCTGGCGA
ATATCGCAGCGCAGTGGCACGAAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References