Detailed information of TA system TA233396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2294441..2295180
• Replicon: chromosome
• Accession: NZ_CP091486
• Organism: Enterobacter cloacae complex sp. ECL411

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: L3069_RS10970
• Protein ID: WP_038416064.1
• Coordinates: 2294441..2294926 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: L3069_RS10975
• Protein ID: WP_003857131.1
• Coordinates: 2294914..2295180 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3069_RS10945 (L3069_10945)	2289494..2290309	-	816	WP_012695470.1	aminoglycoside O-phosphotransferase APH(3')-Ia	-
L3069_RS10950 (L3069_10950)	2290470..2291438	-	969	WP_013815099.1	IS5-like element IS903B family transposase	-
L3069_RS10955 (L3069_10955)	2291470..2291841	-	372	Protein_2135	IS5/IS1182 family transposase	-
L3069_RS10960 (L3069_10960)	2291916..2292548	+	633	WP_001567369.1	hypothetical protein	-
L3069_RS10965 (L3069_10965)	2292577..2293980	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
L3069_RS10970 (L3069_10970)	2294441..2294926	-	486	WP_038416064.1	GNAT family N-acetyltransferase	Toxin
L3069_RS10975 (L3069_10975)	2294914..2295180	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
L3069_RS10980 (L3069_10980)	2295244..2296173	-	930	WP_038416065.1	LysR family transcriptional regulator	-
L3069_RS10985 (L3069_10985)	2296303..2297691	+	1389	WP_038416066.1	MFS transporter	-
L3069_RS10990 (L3069_10990)	2297670..2298227	-	558	WP_023300131.1	OmpH family outer membrane protein	-
L3069_RS10995 (L3069_10995)	2298348..2299484	-	1137	WP_038416067.1	type 1 fimbrial protein	-
L3069_RS11000 (L3069_11000)	2299508..2300086	-	579	WP_022648146.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17546.28 Da
• Isoelectric Point: 9.9308

>T233396 WP_038416064.1 NZ_CP091486:c2294926-2294441 [Enterobacter cloacae complex sp. ECL411]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALAAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGKGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T233396 NZ_HG916765:c1086091-1085837 [Castellaniella defragrans 65Phen]
ATGAAAATCCGGCATCGCAAGACCCTGGCGCCCATCTTCTCCAAACCCATCCCTGCAGGGGTGAAATGGGCCGACGCCAA
GGCGCTGCTGATCGAACTGGGCACGGAGATCACCGAGTGCGAAGGTTCCCGCGTCTCGATCTTCCTGTTCGGGCAGGTGA
GGGTCATGCACCGGCCCCATCCCTCGCCGGACATGGACAAGGGCGCCATCGCCTCCCTGCGCCGGTGGCCCGCAGCACAC
GGAGTACGGCCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT233396 WP_003857131.1 NZ_CP091486:c2295180-2294914 [Enterobacter cloacae complex sp. ECL411]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT233396 NZ_HG916765:c1085840-1085490 [Castellaniella defragrans 65Phen]
ATGAAAAACGTCATGACGATCGGCGGCCACCAGGCCGCCATCATCTACGATCCGGACCTGGAAATGTTCCGCGGCGAATT
CGTCGGGCTCAACGGCGGAGCCGATTTCTATGCGGCCGACATCGCCGGACTGCGCCAGGAGGGCGAGCAGTCGCTCCAGG
TCTTCCTGGACGAATGCAGGCGGCGCGGGATCGAGCCGCGCAAGCACTACTCCGGCCGGTTCGTGCTGCGCGTCGCCCCC
GGCCTCCACGAGGCCGCCGCCCTGGCCGCCGCCGCCCGGGGAGAAAGCCTGAACCAATGGGTGTCCGGCATACTCCAGGA
AGCGCTGGAGCACGCCCACGCCCCGGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9