Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1080942..1081563 Replicon chromosome
Accession NZ_CP091486
Organism Enterobacter cloacae complex sp. ECL411

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag L3069_RS05130 Protein ID WP_015571250.1
Coordinates 1080942..1081160 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag L3069_RS05135 Protein ID WP_006809850.1
Coordinates 1081189..1081563 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3069_RS05100 (L3069_05100) 1076954..1077214 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
L3069_RS05105 (L3069_05105) 1077217..1077357 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
L3069_RS05110 (L3069_05110) 1077354..1078064 - 711 WP_038415530.1 GNAT family protein -
L3069_RS05115 (L3069_05115) 1078167..1079627 + 1461 WP_038415532.1 PLP-dependent aminotransferase family protein -
L3069_RS05120 (L3069_05120) 1079599..1080066 - 468 WP_022647269.1 YlaC family protein -
L3069_RS05125 (L3069_05125) 1080183..1080734 - 552 WP_022647270.1 maltose O-acetyltransferase -
L3069_RS05130 (L3069_05130) 1080942..1081160 - 219 WP_015571250.1 HHA domain-containing protein Toxin
L3069_RS05135 (L3069_05135) 1081189..1081563 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
L3069_RS05140 (L3069_05140) 1082073..1085219 - 3147 WP_023299464.1 multidrug efflux RND transporter permease subunit AcrB -
L3069_RS05145 (L3069_05145) 1085242..1086435 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T233395 WP_015571250.1 NZ_CP091486:c1081160-1080942 [Enterobacter cloacae complex sp. ECL411]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T233395 NZ_HG916765:c1065066-1064884 [Castellaniella defragrans 65Phen]
ATGAACAGCAAAGACCTCATCAAACAACTTGAACAGGCCGGATGGCGATTGCAGAACGTCAAAGGCTCACATCACGTGTA
CAGACATCCAGAACTGCCTGGCCATATCAGCGTGCCACATCCCAAAAAGGCTTTAGGCATCGGACTCGTCACCAAGCTGC
TTCGTCACGCTGGCATGAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT233395 WP_006809850.1 NZ_CP091486:c1081563-1081189 [Enterobacter cloacae complex sp. ECL411]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT233395 NZ_HG916765:c1064873-1064481 [Castellaniella defragrans 65Phen]
ATGAAATACCCCATTGCCATCGAGCCCGGCAACGACACCTCCGCCTGGGGGGTCGTGGTGCCTGACTTGCCCGGCTGCTT
CTCCGCCGCCGACAACGGTATCGACGAAGCCATCGAGAACGCCAAAGAGGCGATCAGCTTGTGGATAGAAGACGCCATCG
CCCACAAAGAGGCCATCCCCAGGCCCTCCAGCATCACCGATCTGCAGCAGTCTGGTGAATACGACGGATGGATATGGGCA
ATCGCCGAGGTCGACCCGGCCCTGATCGACGACACGATCGAACGCGTGAACATCACCCTGCCCCGTCGCATTCTTGCCCT
GCTGGACCAGCGGGCCAAGTCTGCGGGCGAAACCCGATCCGGCTACATCGCACAGATGACCTTGACGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References