Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 236377..236903 Replicon plasmid unnamed1
Accession NZ_CP091482
Organism Enterobacter cloacae complex sp. ECL414

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag L3068_RS24515 Protein ID WP_000323025.1
Coordinates 236377..236664 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag L3068_RS24520 Protein ID WP_000534858.1
Coordinates 236664..236903 (-) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3068_RS24480 (L3068_24480) 231947..232123 - 177 WP_001371930.1 hypothetical protein -
L3068_RS24485 (L3068_24485) 232634..233578 + 945 WP_000778029.1 DUF5417 domain-containing protein -
L3068_RS24490 (L3068_24490) 233674..234276 + 603 WP_001371889.1 hypothetical protein -
L3068_RS24495 (L3068_24495) 234336..234686 + 351 WP_000743059.1 hypothetical protein -
L3068_RS24500 (L3068_24500) 234733..234936 + 204 WP_001015183.1 hypothetical protein -
L3068_RS24505 (L3068_24505) 235218..235538 + 321 WP_000332796.1 hypothetical protein -
L3068_RS24510 (L3068_24510) 236151..236276 - 126 WP_229020258.1 DUF5431 family protein -
L3068_RS24515 (L3068_24515) 236377..236664 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
L3068_RS24520 (L3068_24520) 236664..236903 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
L3068_RS24525 (L3068_24525) 236928..237032 + 105 Protein_245 protein YdfV -
L3068_RS24530 (L3068_24530) 237166..238089 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
L3068_RS24535 (L3068_24535) 238289..238861 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
L3068_RS24540 (L3068_24540) 239337..240575 - 1239 WP_000219087.1 IS110-like element ISEsa2 family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(2'')-Ia / aadA2 / qacE / sul1 / qnrA1 / blaSHV-12 / tet(A) / dfrA16 / blaCTX-M-9 / mcr-9 - 1..294412 294412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T233394 WP_000323025.1 NZ_CP091482:c236664-236377 [Enterobacter cloacae complex sp. ECL414]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T233394 NZ_HG916765:c1019008-1018826 [Castellaniella defragrans 65Phen]
ATGAACAGCACAGACCTTATCAAGCAACTGAAGGCCGACGGCTGGTATCTGGTGCATACCGTTGGGTCGCATCACCAGTT
CAAGCATCCGACCAAGCCCGGCAAGGTCACGGTGCCCCACCCTAAGAAGGAACTGCCCCTACCGACACAACGCAGCATCC
TCAAACAGGCCGGTCTCCGATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT233394 WP_000534858.1 NZ_CP091482:c236903-236664 [Enterobacter cloacae complex sp. ECL414]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT233394 NZ_HG916765:c1018801-1018412 [Castellaniella defragrans 65Phen]
ATGCTCTATCCGATCCACGTGCACAAGGACCGCGACAGTGCCTACGGCGCGTCCTTCCCCGACTTCCCCGGCTGCTTTGC
CGGCGCCGACGAATTGCAGGATCTGCCCCGCGCCGCCCAGGAAGCCGTCGAGGCGCACTTCCATGGCGAAACCGAAGCGA
TCCCCGGGCCCAGCGCACCCGAAGCCTGGAAAGGTCACGAGGACTTCGAGGGCGGCTTTTGGATGATGGTGGATATCGAC
CTGTCCAAGGTCAACACCAAGGCCGTTCGCCTGAACATCAGCCTGCCGGAAAACCTGGTGCATCGCATCGATGCCATGGC
CAAGGCCCAGCACTTATCGCGCTCCGCCTTCCTGGCAAAAGCAGCCGAGCGAGAAATGGCGGAAACTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References