Detailed information of TA system TA233393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 93827..95450
• Replicon: plasmid unnamed1
• Accession: NZ_CP091482
• Organism: Enterobacter cloacae complex sp. ECL414

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: L3068_RS23750
• Protein ID: WP_000088044.1
• Coordinates: 94125..95450 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: L3068_RS23745
• Protein ID: WP_012477377.1
• Coordinates: 93827..94120 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3068_RS23725 (L3068_23725)	89626..90018	+	393	WP_000341066.1	hypothetical protein	-
L3068_RS23730 (L3068_23730)	90677..91333	+	657	WP_000462754.1	trypsin-like serine protease	-
L3068_RS23735 (L3068_23735)	91536..92033	+	498	WP_000340139.1	membrane protein	-
L3068_RS23740 (L3068_23740)	92038..93426	+	1389	WP_012695445.1	hypothetical protein	-
L3068_RS23745 (L3068_23745)	93827..94120	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
L3068_RS23750 (L3068_23750)	94125..95450	+	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
L3068_RS23755 (L3068_23755)	95511..95717	+	207	WP_000134171.1	hypothetical protein	-
L3068_RS23760 (L3068_23760)	95818..96228	+	411	WP_000985909.1	hypothetical protein	-
L3068_RS23765 (L3068_23765)	96241..96669	+	429	Protein_93	hypothetical protein	-
L3068_RS23770 (L3068_23770)	96946..97950	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(2'')-Ia / aadA2 / qacE / sul1 / qnrA1 / blaSHV-12 / tet(A) / dfrA16 / blaCTX-M-9 / mcr-9	-	1..294412	294412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T233393 WP_000088044.1 NZ_CP091482:94125-95450 [Enterobacter cloacae complex sp. ECL414]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T233393 NZ_HG916765:610675-610965 [Castellaniella defragrans 65Phen]
GTGAAGGTCGAGTGGTCGGAGTTTGCGATGGAAGACCGCGACAGGATTTTCGATTACATCGAGTCGGAAAGCCCCAAAGC
AGCGGTATTGGTTGACAGTCGTATCGAAGAACAGGTCAATCGGCTTGCACAGTACCCGGAGTTCGGCCGGCTTGGCCGCG
TTGAGGACACCCGAGAACTGGTCATCGGTCAGACGCCCTATATCGCGCCTTATCGCATCTATGGGGATGTCGTGCGCATC
CTGCGCGTGCTGCATGGGGCGCAACAATGGCCGGATGATTTTGGTGATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT233393 WP_012477377.1 NZ_CP091482:93827-94120 [Enterobacter cloacae complex sp. ECL414]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT233393 NZ_HG916765:610373-610678 [Castellaniella defragrans 65Phen]
ATGTCCGCCAGTGCATTGGTTCAAACTCGCATCGACCCCGTTCTGAAAGAACGGGCCACGGAAGTGCTCAACAACATGGG
CCTCACCGTGTCGGATGCTGTGCGTATTCTATTGACCCGTGTGGCCAATGAAGGGACGCTGCCGTTCAACTTTGCCGTTG
ATCCGGCGGCGCACGACGCCTGGTTCCGCGCCAAGGTTCAAGAGGCCTTGAGCGACCCGCGTCCTGCCGTGTCCAACGAA
GAGGCCTCTGCCCATTTCGCCAAACGCCGCGCCGCCGCCATGCGCAAAGCCGAGGGCAAGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7