Detailed information of TA system TA233391

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4068968..4069647
• Replicon: chromosome
• Accession: NZ_CP091481
• Organism: Enterobacter cloacae complex sp. ECL414

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: R8WLP2
• Locus tag: L3068_RS19520
• Protein ID: WP_006812012.1
• Coordinates: 4068968..4069309 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: R8WMM2
• Locus tag: L3068_RS19525
• Protein ID: WP_006812013.1
• Coordinates: 4069330..4069647 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3068_RS19500 (L3068_19500)	4064242..4065987	+	1746	WP_014885284.1	DNA primase	-
L3068_RS19505 (L3068_19505)	4066153..4067997	+	1845	WP_014885285.1	RNA polymerase sigma factor RpoD	-
L3068_RS19510 (L3068_19510)	4068099..4068605	-	507	WP_014885286.1	G/U mismatch-specific DNA glycosylase	-
L3068_RS19520 (L3068_19520)	4068968..4069309	-	342	WP_006812012.1	TA system toxin CbtA family protein	Toxin
L3068_RS19525 (L3068_19525)	4069330..4069647	-	318	WP_006812013.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L3068_RS19530 (L3068_19530)	4069667..4069888	-	222	WP_001548171.1	DUF987 domain-containing protein	-
L3068_RS19535 (L3068_19535)	4069897..4070373	-	477	WP_000811693.1	RadC family protein	-
L3068_RS19540 (L3068_19540)	4070389..4070847	-	459	WP_205920785.1	antirestriction protein	-
L3068_RS19545 (L3068_19545)	4070945..4071184	-	240	WP_180208556.1	DUF905 domain-containing protein	-
L3068_RS19550 (L3068_19550)	4071261..4071728	-	468	WP_001547765.1	protein YkfB	-
L3068_RS19555 (L3068_19555)	4071751..4072194	-	444	WP_001547764.1	lipoprotein YafY	-
L3068_RS19560 (L3068_19560)	4072194..4072430	-	237	WP_001144031.1	protein YpjK	-
L3068_RS19565 (L3068_19565)	4072471..4073172	-	702	WP_117582372.1	WYL domain-containing protein	-
L3068_RS19570 (L3068_19570)	4073389..4074210	-	822	WP_001548153.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12989.07 Da
• Isoelectric Point: 10.4476

>T233391 WP_006812012.1 NZ_CP091481:c4069309-4068968 [Enterobacter cloacae complex sp. ECL414]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T233391 NZ_HG813242:c1739002-1738793 [Staphylococcus epidermidis PM221]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.45 Da
• Isoelectric Point: 7.0166

>AT233391 WP_006812013.1 NZ_CP091481:c4069647-4069330 [Enterobacter cloacae complex sp. ECL414]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT233391 NZ_HG813242:c1739302-1739009 [Staphylococcus epidermidis PM221]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Toxin

• AlphaFold DB: R8WLP2

Antitoxin

• AlphaFold DB: R8WMM2