Detailed information of TA system TA233389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3016804..3017394
• Replicon: chromosome
• Accession: NZ_CP091481
• Organism: Enterobacter cloacae complex sp. ECL414

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A8B2Y1G2
• Locus tag: L3068_RS14575
• Protein ID: WP_045134957.1
• Coordinates: 3016804..3017136 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: L3068_RS14580
• Protein ID: WP_117581268.1
• Coordinates: 3017137..3017394 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3068_RS14560 (L3068_14560)	3012968..3014293	+	1326	WP_047344763.1	SidA/IucD/PvdA family monooxygenase	-
L3068_RS14565 (L3068_14565)	3014320..3016509	+	2190	WP_045281560.1	TonB-dependent siderophore receptor	-
L3068_RS14575 (L3068_14575)	3016804..3017136	-	333	WP_045134957.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L3068_RS14580 (L3068_14580)	3017137..3017394	-	258	WP_117581268.1	antitoxin	Antitoxin
L3068_RS14585 (L3068_14585)	3018005..3019090	+	1086	WP_232947865.1	hypothetical protein	-
L3068_RS14590 (L3068_14590)	3019201..3021903	+	2703	WP_232947866.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11785.63 Da
• Isoelectric Point: 10.5834

>T233389 WP_045134957.1 NZ_CP091481:c3017136-3016804 [Enterobacter cloacae complex sp. ECL414]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLDGAGTKTMGAIRCDQPRTI
DMAARSGKRLERIPDAVVNEVLARLDAILN

Nucleotide

• Length: 333 bp

>T233389 NZ_HG813242:c1563425-1563246 [Staphylococcus epidermidis PM221]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9427.90 Da
• Isoelectric Point: 4.4488

>AT233389 WP_117581268.1 NZ_CP091481:c3017394-3017137 [Enterobacter cloacae complex sp. ECL414]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT233389 NZ_HG813242:c1564048-1563449 [Staphylococcus epidermidis PM221]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAATTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAAGCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Structures

Toxin

• AlphaFold DB: A0A8B2Y1G2