Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1101701..1102321 Replicon chromosome
Accession NZ_CP091481
Organism Enterobacter cloacae complex sp. ECL414

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag L3068_RS05075 Protein ID WP_014882802.1
Coordinates 1101701..1101919 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag L3068_RS05080 Protein ID WP_008499288.1
Coordinates 1101947..1102321 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3068_RS05045 (L3068_05045) 1097710..1097970 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
L3068_RS05050 (L3068_05050) 1097973..1098113 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
L3068_RS05055 (L3068_05055) 1098110..1098820 - 711 WP_032637309.1 GNAT family protein -
L3068_RS05060 (L3068_05060) 1098922..1100382 + 1461 WP_117581149.1 PLP-dependent aminotransferase family protein -
L3068_RS05065 (L3068_05065) 1100354..1100821 - 468 WP_014882800.1 YlaC family protein -
L3068_RS05070 (L3068_05070) 1100940..1101491 - 552 WP_014882801.1 maltose O-acetyltransferase -
L3068_RS05075 (L3068_05075) 1101701..1101919 - 219 WP_014882802.1 HHA domain-containing protein Toxin
L3068_RS05080 (L3068_05080) 1101947..1102321 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
L3068_RS05085 (L3068_05085) 1102833..1105979 - 3147 WP_014882803.1 multidrug efflux RND transporter permease subunit AcrB -
L3068_RS05090 (L3068_05090) 1106002..1107195 - 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T233379 WP_014882802.1 NZ_CP091481:c1101919-1101701 [Enterobacter cloacae complex sp. ECL414]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T233379 NZ_HG813240:c3104770-3104414 [Mycobacterium tuberculosis 49-02]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT233379 WP_008499288.1 NZ_CP091481:c1102321-1101947 [Enterobacter cloacae complex sp. ECL414]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT233379 NZ_HG813240:c3104984-3104754 [Mycobacterium tuberculosis 49-02]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References