Detailed information of TA system TA233377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 457553..458148
• Replicon: chromosome
• Accession: NZ_CP091481
• Organism: Enterobacter cloacae complex sp. ECL414

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: L3068_RS02155
• Protein ID: WP_117581613.1
• Coordinates: 457798..458148 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: A0A8B2XPR3
• Locus tag: L3068_RS02150
• Protein ID: WP_032637647.1
• Coordinates: 457553..457804 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3068_RS02140 (L3068_02140)	453220..456996	+	3777	WP_117581611.1	autotransporter assembly complex protein TamB	-
L3068_RS02145 (L3068_02145)	456999..457343	+	345	WP_014882267.1	gamma-glutamylcyclotransferase	-
L3068_RS02150 (L3068_02150)	457553..457804	+	252	WP_032637647.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L3068_RS02155 (L3068_02155)	457798..458148	+	351	WP_117581613.1	type II toxin-antitoxin system ChpB family toxin	Toxin
L3068_RS02160 (L3068_02160)	458226..458756	-	531	WP_008501423.1	inorganic diphosphatase	-
L3068_RS02165 (L3068_02165)	459066..460022	+	957	WP_014882271.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
L3068_RS02170 (L3068_02170)	460125..461627	+	1503	WP_045134407.1	sugar ABC transporter ATP-binding protein	-
L3068_RS02175 (L3068_02175)	461638..462663	+	1026	WP_014882273.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-115)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12363.34 Da
• Isoelectric Point: 7.1343

>T233377 WP_117581613.1 NZ_CP091481:457798-458148 [Enterobacter cloacae complex sp. ECL414]
MVKRPCFERGDIVLVGFDPANGHEQKGAGRPALVLSVSAFNQLGMTLVAPVTQGGNFARYAGFSVPLSCEEGNIHGVILV
NQIRMMDLGARLAKRIGVASDETVEDALLRLQAILS

Nucleotide

• Length: 351 bp

>T233377 NZ_HG813240:c3064833-3064417 [Mycobacterium tuberculosis 49-02]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9032.36 Da
• Isoelectric Point: 4.2517

>AT233377 WP_032637647.1 NZ_CP091481:457553-457804 [Enterobacter cloacae complex sp. ECL414]
MRITIKKWGNSAGMVIPGVVMKELGLKPGQSMEAQVTNNQLVLTPVSKQYSLEELLAQCDMSAPEINEQDAWGTSGPVGD
EVW

Nucleotide

• Length: 252 bp

>AT233377 NZ_HG813240:c3065096-3064830 [Mycobacterium tuberculosis 49-02]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A8B2XPR3