Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 233139..233781 Replicon chromosome
Accession NZ_CP091476
Organism Bacillus cereus strain GSICC 30237

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L2I54_RS01320 Protein ID WP_000635963.1
Coordinates 233431..233781 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L2I54_RS01315 Protein ID WP_000004570.1
Coordinates 233139..233426 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2I54_RS01290 (L2I54_01290) 228456..229418 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
L2I54_RS01295 (L2I54_01295) 229411..229983 - 573 WP_061456689.1 rhomboid family intramembrane serine protease -
L2I54_RS01300 (L2I54_01300) 230077..230436 + 360 WP_000583416.1 holo-ACP synthase -
L2I54_RS01305 (L2I54_01305) 230593..231543 + 951 WP_002180340.1 outer membrane lipoprotein carrier protein LolA -
L2I54_RS01310 (L2I54_01310) 231661..232830 + 1170 WP_000390615.1 alanine racemase -
L2I54_RS01315 (L2I54_01315) 233139..233426 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L2I54_RS01320 (L2I54_01320) 233431..233781 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L2I54_RS01325 (L2I54_01325) 233849..236017 + 2169 WP_061456688.1 Tex family protein -
L2I54_RS01330 (L2I54_01330) 236075..236191 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L2I54_RS01335 (L2I54_01335) 236387..236845 + 459 WP_000344234.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T233374 WP_000635963.1 NZ_CP091476:233431-233781 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T233374 NZ_HG813240:c2865553-2865158 [Mycobacterium tuberculosis 49-02]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT233374 WP_000004570.1 NZ_CP091476:233139-233426 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT233374 NZ_HG813240:c2865795-2865550 [Mycobacterium tuberculosis 49-02]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References