Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21570..22213 Replicon plasmid pKO_3
Accession NZ_CP091473
Organism Klebsiella michiganensis strain KO_408

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag L3249_RS30410 Protein ID WP_000754566.1
Coordinates 21570..21986 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag L3249_RS30415 Protein ID WP_001261276.1
Coordinates 21983..22213 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3249_RS30390 (L3249_30420) 16674..18749 + 2076 WP_047929053.1 protease Lon-related BREX system protein BrxL -
L3249_RS30395 (L3249_30425) 18857..19135 - 279 Protein_12 IS3 family transposase -
L3249_RS30400 (L3249_30430) 19152..20228 - 1077 WP_000227970.1 IS110 family transposase -
L3249_RS30405 (L3249_30435) 20510..21366 - 857 Protein_14 IS3-like element ISEc15 family transposase -
L3249_RS30410 (L3249_30440) 21570..21986 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
L3249_RS30415 (L3249_30445) 21983..22213 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L3249_RS30420 (L3249_30450) 22802..23095 + 294 WP_020956883.1 hypothetical protein -
L3249_RS30425 (L3249_30455) 23159..23845 + 687 WP_020956882.1 hypothetical protein -
L3249_RS30430 (L3249_30460) 23857..24633 + 777 WP_020956881.1 tyrosine-type recombinase/integrase -
L3249_RS30435 (L3249_30465) 24691..24948 - 258 WP_000764642.1 hypothetical protein -
L3249_RS30440 (L3249_30470) 25077..25181 - 105 WP_032409716.1 hypothetical protein -
L3249_RS30445 (L3249_30475) 25711..26577 + 867 WP_004118283.1 replication initiation protein -
L3249_RS30450 (L3249_30480) 26754..27023 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / tet(A) - 1..53503 53503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T233373 WP_000754566.1 NZ_CP091473:c21986-21570 [Klebsiella michiganensis]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T233373 NZ_HG813240:2864291-2864668 [Mycobacterium tuberculosis 49-02]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT233373 WP_001261276.1 NZ_CP091473:c22213-21983 [Klebsiella michiganensis]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT233373 NZ_HG813240:2864037-2864294 [Mycobacterium tuberculosis 49-02]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References