Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 87243..88866 Replicon plasmid pKO_1
Accession NZ_CP091471
Organism Klebsiella michiganensis strain KO_408

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag L3249_RS28890 Protein ID WP_000088046.1
Coordinates 87541..88866 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag L3249_RS28885 Protein ID WP_024136327.1
Coordinates 87243..87536 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3249_RS28860 (L3249_28890) 83115..84356 + 1242 WP_238076264.1 VWA domain-containing protein -
L3249_RS30940 84447..84578 + 132 WP_000374059.1 hypothetical protein -
L3249_RS28865 (L3249_28895) 84919..85200 - 282 WP_000077926.1 hypothetical protein -
L3249_RS28870 (L3249_28900) 85250..85441 - 192 WP_000398480.1 hypothetical protein -
L3249_RS28875 (L3249_28905) 85533..85874 - 342 WP_001151575.1 hypothetical protein -
L3249_RS28880 (L3249_28910) 86247..86639 + 393 WP_000341066.1 hypothetical protein -
L3249_RS28885 (L3249_28915) 87243..87536 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
L3249_RS28890 (L3249_28920) 87541..88866 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
L3249_RS28895 (L3249_28925) 88927..89133 + 207 WP_000134171.1 hypothetical protein -
L3249_RS28900 (L3249_28930) 89235..89645 + 411 WP_000985911.1 hypothetical protein -
L3249_RS28905 (L3249_28935) 89658..90473 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
L3249_RS28910 (L3249_28940) 90727..91152 + 426 WP_001043843.1 hypothetical protein -
L3249_RS28915 (L3249_28945) 91701..92009 + 309 WP_001224687.1 hypothetical protein -
L3249_RS28920 (L3249_28950) 92025..92882 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
L3249_RS28925 (L3249_28955) 92944..93147 + 204 WP_001194555.1 hypothetical protein -
L3249_RS28930 (L3249_28960) 93489..93845 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / floR / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / dfrA12 - 1..286463 286463


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T233372 WP_000088046.1 NZ_CP091471:87541-88866 [Klebsiella michiganensis]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T233372 NZ_HG813240:2863585-2863998 [Mycobacterium tuberculosis 49-02]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT233372 WP_024136327.1 NZ_CP091471:87243-87536 [Klebsiella michiganensis]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT233372 NZ_HG813240:2864037-2864294 [Mycobacterium tuberculosis 49-02]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References