Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4587844..4588463 Replicon chromosome
Accession NZ_CP091470
Organism Klebsiella michiganensis strain KO_408

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag L3249_RS21830 Protein ID WP_004099646.1
Coordinates 4588245..4588463 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag L3249_RS21825 Protein ID WP_004129911.1
Coordinates 4587844..4588218 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3249_RS21815 (L3249_21840) 4583001..4584194 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L3249_RS21820 (L3249_21845) 4584217..4587363 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
L3249_RS21825 (L3249_21850) 4587844..4588218 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
L3249_RS21830 (L3249_21855) 4588245..4588463 + 219 WP_004099646.1 HHA domain-containing protein Toxin
L3249_RS21835 (L3249_21860) 4588626..4589192 + 567 WP_195377661.1 maltose O-acetyltransferase -
L3249_RS21840 (L3249_21865) 4589164..4589298 - 135 WP_223226764.1 hypothetical protein -
L3249_RS21845 (L3249_21870) 4589319..4589789 + 471 WP_014228205.1 YlaC family protein -
L3249_RS21850 (L3249_21875) 4589764..4591218 - 1455 WP_014837606.1 PLP-dependent aminotransferase family protein -
L3249_RS21855 (L3249_21880) 4591320..4592018 + 699 WP_195377660.1 GNAT family protein -
L3249_RS21860 (L3249_21885) 4592015..4592155 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
L3249_RS21865 (L3249_21890) 4592155..4592418 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T233370 WP_004099646.1 NZ_CP091470:4588245-4588463 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T233370 NZ_HG813240:2803747-2804172 [Mycobacterium tuberculosis 49-02]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGCGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT233370 WP_004129911.1 NZ_CP091470:4587844-4588218 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT233370 NZ_HG813240:2803520-2803741 [Mycobacterium tuberculosis 49-02]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References