Detailed information of TA system TA233361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2030841..2031431
• Replicon: chromosome
• Accession: NZ_CP091470
• Organism: Klebsiella michiganensis strain KO_408

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: L3249_RS09645
• Protein ID: WP_195378294.1
• Coordinates: 2031099..2031431 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: J6I3K7
• Locus tag: L3249_RS09640
• Protein ID: WP_004852307.1
• Coordinates: 2030841..2031098 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3249_RS09615 (L3249_09630)	2026098..2026703	-	606	WP_014838893.1	glutathione S-transferase family protein	-
L3249_RS09620 (L3249_09635)	2026883..2027809	+	927	WP_014838892.1	LysR substrate-binding domain-containing protein	-
L3249_RS09625 (L3249_09640)	2027847..2028845	-	999	WP_238076208.1	aldo/keto reductase	-
L3249_RS09630 (L3249_09645)	2028946..2029860	+	915	WP_195378295.1	LysR family transcriptional regulator	-
L3249_RS09635 (L3249_09650)	2029979..2030248	-	270	WP_195378322.1	hypothetical protein	-
L3249_RS09640 (L3249_09655)	2030841..2031098	+	258	WP_004852307.1	antitoxin	Antitoxin
L3249_RS09645 (L3249_09660)	2031099..2031431	+	333	WP_195378294.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L3249_RS09650 (L3249_09665)	2031934..2033121	+	1188	WP_195378293.1	nucleoid-associated protein	-
L3249_RS09655 (L3249_09670)	2033147..2034298	+	1152	WP_227631862.1	hypothetical protein	-
L3249_RS09660 (L3249_09675)	2034376..2035230	-	855	WP_195378291.1	hypothetical protein	-
L3249_RS09665 (L3249_09680)	2035434..2036308	-	875	Protein_1891	integrase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2021137..2036308	15171

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11900.88 Da
• Isoelectric Point: 10.8850

>T233361 WP_195378294.1 NZ_CP091470:2031099-2031431 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVPLEGAGIKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILR

Nucleotide

• Length: 333 bp

>T233361 NZ_HG813240:2188024-2188401 [Mycobacterium tuberculosis 49-02]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9422.88 Da
• Isoelectric Point: 4.4607

>AT233361 WP_004852307.1 NZ_CP091470:2030841-2031098 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT233361 NZ_HG813240:2188443-2188892 [Mycobacterium tuberculosis 49-02]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCATCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3HDW8